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Application of small molecule compound in promoting growth and development of plants

A small molecule compound, a technology to promote plant growth, applied in the direction of plant growth regulator, plant growth regulator, application, etc., can solve the problem that tissue or organ specificity cannot be achieved

Active Publication Date: 2021-05-11
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, the present invention has discovered a small molecular compound through research, which has tissue or organ specificity to the growth and development of plants, and uses the distribution mechanism of plant endogenous auxin in growth and development to balance the effects of various tissues and organs on the growth and development of plants. The need for auxin, addressing the inability of current auxin-based products to achieve tissue or organ specificity

Method used

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  • Application of small molecule compound in promoting growth and development of plants
  • Application of small molecule compound in promoting growth and development of plants
  • Application of small molecule compound in promoting growth and development of plants

Examples

Experimental program
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specific Embodiment

[0062] The following will be described in detail in conjunction with specific embodiments. The following examples do not include other components except unavoidable impurities unless otherwise specified. The reagents and instruments used in the examples are all conventional choices in the art unless otherwise specified. The experimental methods for which specific conditions are not indicated in the examples are implemented according to conventional conditions, such as the conditions described in literature, books or the method recommended by the manufacturer.

[0063] It should be noted that, in the following examples, the structure is The small molecular compound is called "C9" for short. C9 is synthesized by Shanghai Nafu Biological Co., Ltd., and it is dissolved in DMSO and added to the medium when used. In this article, "μM" refers to "μmol / L"; PPBo refers to 4-phenoxyphenylboronic acid; L-Kyn refers to L-Kynurenine, an inhibitor of the auxin synthesis pathway IPA pathw...

Embodiment 1

[0065] Effects of Different Concentrations of C9 on Taproot Length, Hypocotyl Length and Adventitious Root Number of Arabidopsis

[0066] (1) Seeds of Arabidopsis thaliana (Columbia type 0, Col-0) were sown on the medium of C9 group, IAA group and blank control group, cultured for 6 days, and at least 30 seeds were sown in each group. Among them, the culture medium of the C9 group contained 0.1 μM, 0.3 μM, 0.8 μM, 1.5 μM, 3 μM and 5 μM C9 respectively. Culture medium; the culture medium of IAA group was containing 0.05μM, 0.1μM, 0.3μM and 1μM IAA respectively culture medium; the culture medium of the blank control group was not treated Medium. Certainly, other culture conditions of C9 group, IAA group and blank control group are the same.

[0067] (2) After the cultivation, observe the growth status of the Arabidopsis seedlings in each group, record the main root length, hypocotyl length and the number of adventitious roots of the Arabidopsis seedlings treated with diffe...

Embodiment 2

[0074] Effect of C9 on the number of lateral roots in Arabidopsis

[0075] (1) will be in Arabidopsis thaliana seedlings (Columbia type 0, Col-0) grown on medium for 5 days with consistent growth were randomly divided into three groups: C9 group, IAA group and blank control group.

[0076] (2) Transfer the Arabidopsis seedlings of the C9 group to the culture medium containing 0.8 μM and 1.5 μM C9 The treated plates of medium were cultured for 3 days. Similarly, the Arabidopsis seedlings of the IAA group were transferred to the The treated plates of medium were cultured for 3 days. The Arabidopsis seedlings of the blank control group were only transferred to the Cultured on medium (without additional addition of C9, IAA or other substances to the treated plates) for 3 days. Certainly, other culture conditions of C9 group, IAA group and blank control group are the same. After the cultivation, the plant phenotype and the number of lateral roots of the Arabidopsis seedlin...

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Abstract

The invention relates to application of a small molecule compound in promoting plant growth and development. The structural formula of the small molecule compound is shown in the specification, and the small molecule compound can inhibit amino acid of plant endogenous auxin by inhibiting the activity of plant GH3 protein, so that accumulation of the auxin in a plant shows original tissue or organ specificity, and growth and development of the plant are facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a GH3 protein inhibitor in regulating plant growth and development. Background technique [0002] Auxin is a class of endogenous hormones containing an unsaturated aromatic ring and an acetic acid side chain, and its chemical essence is indole acetic acid. Indole-3-acetic acid (IAA) is the most important form of auxin in plants. It regulates many aspects of plant growth and development, such as regulation of plant cell division and elongation, plant tropism and apical dominance, lateral root development and adventitious root development. formation, leaf abscission, and fruit development. The regulation of auxin mainly depends on the concentration of auxin, too high or too low auxin concentration will have adverse effects on plants. Therefore, the research on the regulation of plant auxin concentration is of great significance for the application of agricultural p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/58A01P21/00A01G24/30
CPCA01N43/58A01G24/30
Inventor 郭红卫谢银鹏姜凯朱颖
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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