Method for improving methane fermentation activity of methanol and application of method

A methane fermentation and methanol technology, applied in fermentation, chemical instruments and methods, and adding compounds to stimulate growth, etc., can solve the problems of poor methanol wastewater treatment efficiency and toxicity, improve economy and effectiveness, and increase methane production. rate, and the effect of improving fermentation activity

Active Publication Date: 2021-05-28
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although methanol has high biodegradability, high concentration methanol has certain toxicity to microorganisms, which makes the treatment efficiency of high concentration methanol wastewater poor

Method used

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  • Method for improving methane fermentation activity of methanol and application of method
  • Method for improving methane fermentation activity of methanol and application of method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The inoculum was taken from anaerobic activated sludge in an upflow anaerobic sludge bed reactor with an organic load of 5 kg / (m 3 ·d), the daily volumetric gas production rate is 2.1L / (L·d).

[0035] Basic anaerobic medium formula: add methanol 10g, NaHCO to 1L deionized water 3 4g, Na 2 HPO 4 2H 2 O530mg, KH 2 PO 4 410mg, NH 4 Cl 300mg, CaCl 2 2H 2 O 110mg, MgCl 2 ·6H 2 O 100mg, NaCl 300mg, FeCl 2 4H 2 O 4.5mg, EDTA Na 2 1.65mg, trace element solution 1mL, vitamin solution 1mL.

[0036] Formula of trace element solution (mg / L): H 3 BO 4 50, ZnCl 2 50, CuCl 2 ·H 2 O 38 , MnCl 2 4H 2 O50, CoCl 2 ·6H 2 O 50, NiCl 2 ·6H 2 O 92,Na 2 SeO 3 ·5H 2 O26, Na 2 WO 4 2H 2 O 33 , Na 2 MoO 4 2H 2 O 24.

[0037] Vitamin liquid formula (mg / L): biotin 4, niacin 40, vitamin B610, vitamin B220, vitamin B140, vitamin B1220, folic acid 20, lipoic acid 40, p-aminobenzoic acid 20.

[0038] The basic anaerobic medium preparation method is as follows (1L): we...

Embodiment 2

[0050] In this example, C 6 -HSL was directly added to the influent of methane fermentation reactor for single factor control experiment.

[0051] Two upflow anaerobic sludge bed reactors with an effective volume of 4L were selected, and the influent water was diluted with methanol to a COD of 6000mg / L, and sodium bicarbonate was added to adjust the alkalinity to 3000mg / L (as CaCO 3 The inoculum sludge was mature anaerobic granular sludge, and the inoculum volume was 1L.

[0052] Add C at a final concentration of 5 μg / L to the influent of reactor R1 6 -HSL, reactor R2 is the control, no C is added to the influent 6 -HSL. Two upflow anaerobic sludge bed reactors were operated under the conditions of hydraulic retention time of 24h and temperature of 35°C, and the COD values ​​of the effluent from the two reactors were measured respectively.

[0053] Results: The average COD of R1 effluent was 259.61mg / L, and the average COD of R2 effluent was 826.37mg / L.

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Abstract

The invention provides a method for improving methane fermentation activity of methanol, and belongs to the technical field of methane fermentation of organic wastewater. According to the invention, a quorum sensing signal molecule, namely, N-hexanoyl homoserine lactone (C6-HSL), is added into a methanol methane fermentation system to regulate and control a microbial quorum sensing signal system, so that the group metabolic activity of methanogens can be effectively improved, the methanol degradation rate and the methane production rate are further improved, and the economic benefit of methanol wastewater methane fermentation is improved.

Description

technical field [0001] The invention belongs to the technical field of methane fermentation of organic waste water, and in particular relates to a method for improving the fermentation activity of methanol methane and its application. Background technique [0002] Methanol (Methanol, dried, CH 3 OH) is a saturated monohydric alcohol with the simplest structure. As a pillar product in the chemical industry, it is widely used in organic synthesis, petrochemical and forestry chemical production. The resulting wastewater also contains high concentrations of methanol. Methanol is highly toxic to the human body, the lowest dose of oral poisoning is about 100mg / kg (body weight), and oral intake of 0.3-1g / kg (body weight) can be fatal. At the same time, methanol has certain toxicity to microorganisms. Methanol with a concentration of 790mg / L can reduce the degradation efficiency of organic matter in the biofilter, and methanol with a concentration of 5000mg / L can inhibit the digest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/38C02F3/34C02F101/34
CPCC12P5/023C12N1/38C02F3/34C02F2101/34Y02E50/30
Inventor 刘凤琴张玉鹏袁志良陈云刘红恩卢凯歌李畅
Owner HENAN AGRICULTURAL UNIVERSITY
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