50 results about "Methanogenium aggregans" patented technology

The invention discloses methanogen lyase homologous with a PeiR lyase sequence and application of the methanogen lyase, and aims to solve the problem that PeiR lyase protein of a methanogen cell wall peptide bond can participate in a biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the PeiR lyase protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogens virus from rumen microbiome sequencing data, and sequence homology between the proteins and PeiR protein is further analyzed to obtain a series of methanogens lyases. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiW lyase and application of the methanogen lyase, and aims to solve the problem that PeiW lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model between the protein sequence and PeiW is analyzed, and two methanogens lyases similar to the high-dimensional characteristics obtained by the PeiW model are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through detection of a crude enzyme solution, the effectiveness of the lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test.

The invention discloses methanogen lyase with the same protein family annotation as PeiR lyase and application of the methanogen lyase, and the methanogen lyase is obtained through homologous screening of Pfam protein family database annotation based on known PeiR lyase characteristics aiming at methanogen cell wall peptide bonds. The PeiR lyase protein can participate in the biological process of hydrolyzing archaea cell walls, methanogens are effectively killed, methane production is reduced, and the protein homologous with the protein has potential methane reduction potential. According to the invention, by integrating metagenome sequencing data of rumen microorganisms, screening methanogenic bacterium virus protein by using series bioinformatics software, and combining Pfam functional domain annotation to carry out homologous relationship analysis, a series of lyase is finally identified and is successfully expressed in a prokaryotic expression system, and meanwhile, an in-vitro gas production experiment result also shows that the methanogenic bacterium virus protein can be successfully expressed in a prokaryotic expression system. The crude enzyme liquid can significantly reduce the methane generation amount.

The present invention relates to polypeptide and peptide vaccine compositions and methods that reduce methane and / or hydrogen production in animals. The present invention also relates to the treatment of diseases that are associated with methanogens.

The present invention relates to polypeptide and peptide vaccine compositions and methods that reduce methane and / or hydrogen production in animals. The present invention also relates to the treatment of diseases that are associated with methanogens.

The present invention relates to nucleic acid vaccine compositions and methods that target methanogens and reduce their production of methane and / or hydrogen. The present invention also relates to the treatment of diseases that are associated with methanogens.

The invention provides a microbial agent and method for cooperating with efficient degradation of chlorinated organics and controlling methane emission, and belongs to the technical field of chlorinated organics degradation, the method comprises the following steps: 1) mixing dechlorination flora with methanogens to obtain a microbial agent; (2) degrading chlorinated organic matters by using the microbial agent; the dechlorination flora contains bacillus subtilis of which the cell number accounts for more than 80%; the cell number ratio of the bacillus subtilis to the methanogens is (10-50): 1. By precisely regulating and controlling the ratio of the dechlorination bacteria to the methanogens, the degradation efficiency of COPs can be effectively improved, meanwhile, the generation amount of methane is reduced, and the synergistic interaction of pollution reduction and carbon reduction is achieved.

The invention relates to a novel methanogen inhibitor for ruminants. The invention also extends to the use of such compounds in ruminants to reduce methane production in the rumen and / or to increase productivity in ruminants.

This invention discloses methanogenic lyases with the same protein family annotation as PeiR lyases and their applications. These enzymes are obtained through homology screening based on the known characteristics of PeiR lyases targeting peptide bonds in the methanogenic cell wall, using the Pfam protein family database annotation. PeiR lyase proteins participate in the biological process of hydrolyzing archaea cell walls, effectively killing methanogens and reducing methane production. Proteins homologous to this protein have potential methane-reducing potential. This invention integrates rumen microbial metagenomic sequencing data, uses a series of bioinformatics software to screen methanogenic viral proteins, and combines Pfam functional domain annotation for homology analysis, ultimately identifying a series of lyases. These lyases were successfully expressed in a prokaryotic expression system, and in vitro gas production experiments also showed that the crude enzyme solution significantly reduced methane production.

The invention discloses a pharmaceutical composition for reducing methane emission of ruminants and improving feed digestibility and a preparation method of the pharmaceutical composition, and belongs to the technical field of feed additives. Comprising the following components in parts by weight: 35-45 parts of malt, 30-40 parts of mangnolia officinalis and 25-35 parts of rhizoma atractylodis. During preparation, the malt, the mangnolia officinalis and the rhizoma atractylodis are respectively crushed and sieved, and then the crushed malt, the crushed mangnolia officinalis and the crushed rhizoma atractylodis are fully mixed to obtain the pharmaceutical composition. On one hand, the antibacterial characteristics of the cortex magnoliae officinalis and the rhizoma atractylodis can remodel gastrointestinal tract microflora, the activity of microorganisms closely related to methane generation such as methanogens is accurately inhibited, and methane emission is reduced from the source; and on the other hand, digestive enzymes of malt directly act on feed nutrition decomposition, and magnolia officinalis and rhizoma atractylodis jointly construct an efficient digestion system by maintaining intestinal health and optimizing a digestion environment, so that the feed digestibility is remarkably improved, and growth, development and production performance improvement of ruminants are promoted.

Disclosed herein are polynucleotides encoding one or more rumen-associated antigens, and polypeptides encoded by the polynucleotides. Also provided herein are compositions comprising the polynucleotides, and methods of making and using the compositions.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The present invention relates to cell-based vaccine compositions and methods that reduce methane and / or hydrogen production in animals. The present invention relates to the treatment of diseases that are associated with methanogens. The present invention also relates to methods of growing hydrogenotrophs in safe anaerobic conditions.

The present invention relates to polypeptide and peptide vaccine compositions and methods that reduce methane and / or hydrogen production in animals. The present invention also relates to the treatment of diseases that are associated with methanogens.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiP lyase and application of the methanogen lyase, and aims to solve the problem that PeiP lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model TM-Vec between the protein sequence and PeiP is analyzed, and the methanogens lyase homologous with the PeiP on the high-dimensional feature level of the model is obtained. According to the invention, multiple segments of primers are designed to synthesize a target sequence, the expression of the lyase is successfully realized in a prokaryotic expression system, and the effectiveness of the novel lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test by detecting a crude enzyme solution.

The invention discloses methanogen lyase homologous with a PeiW lyase structure and application of the methanogen lyase homologous with the PeiW lyase structure, and the methanogen lyase is obtained by screening from rumen microbiome sequencing data through a structure comparison method based on known PeiW lyase characteristics aiming at methanogen cell wall peptide bonds. The PeiW lyase protein can participate in the biological process of hydrolyzing the cell wall of methanogens, the methanogens are effectively killed, methane generation is reduced, and the protein homologous with the protein structure has potential methane reduction potential. According to the invention, rumen metagenome data and virome data are integrated, potential protease is screened by using bioinformatics software, and a sequence homologous with a PeiW structure is identified to obtain two novel lyases. A target sequence is synthesized through multiple segments of primers and is expressed in a prokaryotic expression system, and meanwhile, a crude enzyme liquid in-vitro gas production test shows that the lyase can remarkably reduce the methane yield.

The present invention relates to a class of methanogen inhibitors for ruminants. The invention also extends to the use of such compounds in ruminants to reduce methane production in the rumen and / or to enhance productivity in the ruminant.

The invention discloses a microbial flora for treating sulfate wastewater and a preparation method of the microbial flora. Comprising the following microbial treatment flora components: 10-35% of biphasic photosynthetic bacteria, 5-10% of sulfate reducing bacteria, 5-30% of bifidobacterium, 3-8% of desulfurization vibrio, 5-25% of mesophilic actinomycetes, 2-5% of methanogens, 10-30% of sub-digestion bacteria, 2-6% of acidithiobacillus acidophilus, 12-30% of bacillus megatherium, 1-4% of iron-oxidizing bacteria, 1-5% of denitrifying bacteria and 1-3% of phosphorus-accumulating bacteria. According to the method, sulfate is efficiently removed, the removal rate of SO4 < 2-> is larger than or equal to 85%, the H2S output is reduced by 70% or above, multi-pollutant cooperative treatment of odor pollution is avoided, the removal rate of COD is larger than or equal to 60%, the removal rates of total nitrogen and total phosphorus are larger than or equal to 50% and 40% respectively, the comprehensive discharge standard is met, the environmental adaptability is high, the salt tolerance NaCl is smaller than or equal to 5%, the temperature fluctuation resistance is 20-40 DEG C, and the method is suitable for the complex environment of industrial wastewater, low in cost and suitable for industrial production. Extra chemical agents are not needed, strains can be bred autonomously, and the operation cost is reduced by 40% or above compared with a traditional method.

The invention relates to the first hydrolase that specifically digests the archaeal type of a peptidoglycan (PG), the major component of the cell wall of methanogens, named Talosaminidase. The enzyme consists of a signal peptide, several bacterial IG domains, a glycosyl hydrolase, several archaeal PG binding domains and a peptidase domain. The double enzymatic activity for the glycosyl hydrolase and the peptidase maximizes the lysis of archaeal cells with a cell wall of the archaeal PG-type. Engineering the enzyme by removing the signal peptide and bacterial IG domains and keeping only the archaeal PG binding, glycosyl hydrolase and peptidase made the enzyme easier to express and purify, without negatively influencing the enzymatic activity. Therefore, engineered Talosaminidase can be used to regulate populations of archaeal methanogens in the industrial, agricultural and medical settings, and consequently methane production.

The disclosure relates to compositions and methods of bacterial species expressing endolysins for the prophylaxis and treatment of methanogen growth in the digestive tract of an animal. More specifically, the disclosure relates to agents, compositions, and methods for preventing and / or inhibiting growth of methanogens.

The invention relates to the technical field of oil exploitation, and discloses a compound microbial agent as well as a preparation method and application thereof. The compound microbial agent contains methanogens and bacillus. The methanogens are selected from at least one of methanosarcina martensii, methanobacterium formate and methanobacterium thermophilus; the bacillus is selected from at least one of bacillus megatherium, bacillus cereus and bacillus subtilis; the number of viable bacteria of the methanogens in the compound microbial agent is 1 * 10 < 6 > CFU / mL to 1 * 10 < 9 > CFU / mL; the number of viable bacteria of the bacillus in the compound microbial agent is 5 * 10 < 5 > CFU / mL to 5 * 10 < 8 > CFU / mL. The composite microbial agent has excellent carbon sequestration performance, and is suitable for depleted oil and gas reservoirs, deep saline water layers and other geological sequestration scenes.

The invention belongs to the technical field of feed additives, and particularly discloses a composite methane inhibitor and a preparation method thereof. The composite methane inhibitor is prepared from the following raw materials in parts by weight: 16 to 21 parts of oligomeric schizophyllan, 6 to 10 parts of atractylenolide IV, 3 to 5 parts of loganin, 1 to 5 parts of mannosylerythritol lipid and 4 to 8 parts of modified montmorillonite. Experimental results show that all the components of the compound methane inhibitor cooperate with one another, and the effects of adjusting intestinal flora and reducing the methane content can be achieved. Specifically, atractylenolide IV and loganin synergistically inhibit the activity of methanogens, and oligomeric schizophyllan has antioxidant activity, so that oxidation of unsaturated fatty acid can be inhibited, and side reactions possibly promoting generation of methane are reduced; and the modified montmorillonite has relatively large interlayer spacing and specific surface area, has an excellent adsorption effect, and is beneficial to keeping activity of components such as oligomeric schizophyllan and the like, so that the inhibition effect is further enhanced.

The invention discloses methanogen lyase homologous with a PeiP lyase structure and application of the methanogen lyase, and the methanogen lyase is screened from rumen microbiome sequencing data through a structure comparison method based on known PeiP lyase protein characteristics. The PeiP lyase protein can participate in the biological process of hydrolyzing archaea cell walls, methanogens are effectively killed, methane production is reduced, and the protein homologous with the protein has potential methane reduction potential. Protein sequences coded by methanogens viruses are extracted from rumen microbiome sequencing data, the structural similarity between the proteins and PeiP proteins is analyzed by using a bioinformatics tool, and finally two lyases are successfully identified. In order to realize the expression, multiple segments of primers are designed and synthesized to construct a target sequence, and high-efficiency expression is realized in a prokaryotic expression system. In-vitro gas production experiments show that the crude enzyme liquid can significantly reduce methane production.

The present invention relates to a transformant having isoprene-producing ability The transformant of the present invention has superior isoprene-producing ability by introduction, into methanotrophic bacteria, a nucleotide sequence encoding exogenous isoprene synthase, a nucleotide sequence encoding exogenous isopentenyl diphosphate isomerase, and a nucleotide sequence encoding exogenous 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, and thus can be effectively used for isoprene biosynthesis.

The invention discloses an advanced oxidation treatment device and treatment method for printing and dyeing wastewater, and relates to the technical field of sewage treatment.The device comprises a closed reactor, a feeding port is formed in the bottom of the closed reactor, and a discharging port and an exhaust port are formed in the top of the closed reactor; a functional filler layer is arranged in the closed reactor; activating balls and persulfate are arranged in the functional filler layer, the activating balls are formed by soaking Fe3O4 nano-particles and wine brewing wastewater fermentation liquor, and volatile fatty acid adsorbed on the surfaces of the activating balls is at pH gt; when the temperature is 7.5, the persulfate is desorbed and activated to generate sulfate free radicals. According to the invention, an autocatalysis system composed of pH responsive activation balls and persulfate is constructed in the functional filler layer, so that sulfate free radicals can be generated without adding an external agent, and meanwhile, the purpose of reducing carbon emission by inhibiting the activity of methanogens through metabolic competition is achieved.

The present invention belongs to the technical field of feed additives, and specifically discloses a composite methane inhibitor and a preparation method thereof. The composite methane inhibitor comprises the following raw materials by weight: 16-21 parts of oligomeric schizophyllan, 6-10 parts of atractylodes lactone IV, 3-5 parts of loganin, 1-5 parts of mannose erythritol lipids, and 4-8 parts of modified montmorillonite. Experimental results show that the components of the composite methane inhibitor cooperate with each other to achieve the effect of regulating intestinal flora and reducing methane content. Specifically, atractylodes lactone IV and loganin synergistically inhibit the activity of methanogens, and oligomeric schizophyllan can inhibit the oxidation of unsaturated fatty acids due to its antioxidant activity, reducing side reactions that may promote methane production; and modified montmorillonite has a larger interlayer spacing and specific surface area, and has excellent adsorption, which is conducive to maintaining the activity of components such as oligomeric schizophyllan, thereby further enhancing the inhibitory effect.

The present application belongs to the field of biological fermentation, and particularly relates to a purification method for preparing natural gas from biomass, a porous hydrophobic carrier, and a porous particle prepared from the carrier by using a long-chain branched polymer; a hydrophilic modification is performed on the surface of the porous particle to obtain an amphiphilic carrier; then the amphiphilic carrier is stored in a hydrogen atmosphere to obtain a hydrogen-loaded particle; the hydrogen-loaded particle is mixed with an inoculum containing hydrogenotrophic methanogens, and then the mixture is used to ferment the biomass in an aqueous system to produce natural gas, thereby forming purification. The gas prepared by the method has a high content of methane and a low content of carbon dioxide, and is equivalent to natural gas in content, and can be used as natural gas.

The invention discloses methanogen lyase homologous with a PeiR lyase structure and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of bioinformatics software is utilized to identify the protein sequence coded by methanogens virus from a large amount of rumen microorganism virus group sequencing data, and the structural homology between the protein and PeiR is analyzed on the basis. Finally, the invention successfully obtains a series of methanogen lyase homologous with the PeiR structure. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. An in-vitro gas production test of a crude enzyme solution shows that the lyase is effective in the aspect of reducing the yield of methane.

The invention relates to the technical field of feed additives, in particular to a sustained-release feed additive containing eucalyptus globulus essential oil for rumens of cattle and sheep. The CO2-triggered eucalyptus globulus essential oil sustained-release capsule comprises a CO2-triggered eucalyptus globulus essential oil microcapsule unit, a cobalt acetate sustained-release microsphere unit and a carrier unit. Wherein the microcapsule unit is of a three-layer core-shell structure and can respond to CO2 partial pressure in the rumen gas production peak period to achieve pulse type rapid release of eucalyptus globulus essential oil, and methanogens are accurately inhibited; the sustained-release microsphere unit adopts functionalized polysaccharide composite microspheres to load cobalt acetate, can stably release cobalt element in rumen, and slowly and continuously releases cobalt element in abomasum and intestinal tract after passing the rumen. The two parts are compounded in a physical mode and do not interfere with each other, and space-time precise delivery and function coordination at different parts of the digestive tract are achieved. The method can effectively improve the methane emission reduction efficiency, improve rumen fermentation and improve the nutrient digestibility of the feed.

The present disclosure relates to compositions and methods comprising a genetically modified strain of Arthrospira prastrata that expresses a methanogen killer lyase (methanolysin) to inhibit methane emissions produced by the Enterochaea. The present disclosure also relates to methods and compositions comprising a genetically modified strain of Arthrospira prastrata that expresses a methanogen killer lyase (methanolysin) to inhibit methane emissions produced by the Enterochaea.