Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Preparation method and application of mogroside V standard sample

A mogroside and standard sample technology, applied in the field of analytical chemistry, can solve the problems of complex purification difficulty of mogroside V analogs, inability to realize industrialized large-scale production, long delivery period, etc., and achieve stable product quality and preparation speed. Fast, high-volume results

Pending Publication Date: 2021-05-28
SECOND MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no research institution in China that can provide mogroside V standard samples and their preparation methods, and importing mogroside V standard samples from abroad has problems such as high costs, cumbersome procedures, and long delivery cycles
On the other hand, mogroside V analogues are complex and difficult to purify, and relying on direct extraction from Luo Han Guo cannot achieve large-scale industrial production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of mogroside V standard sample
  • Preparation method and application of mogroside V standard sample
  • Preparation method and application of mogroside V standard sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of the test solution: dissolving the industrial-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolving it, and then filtering through a 0.22 μm filter membrane to obtain the test solution;

[0037] (2) Liquid chromatography preparation: inject the solution of the test sample into the preparation chromatography to separate pure fractions, wherein the chromatographic conditions: the chromatographic column is PREP-ODS(H) KIT (250mm×20mm, 5 μm), mobile phase: 23% (v / v) Acetonitrile aqueous solution; flow rate: 5-15mL / min; column temperature: 30°C; running time: 15min; detection wavelength: 202nm, injection volume 0.1-1mL, and collect fractions for 9-11min;

[0038] (3) The drying of the fractions is to freeze-dry in a freeze dryer at -60 to -40°C for 20 to 30 hours, and after the freeze-dried mogroside V solid is ground and pulverized, it is freeze-dried again for 20 to 30 hours to obtain Luo Han Guo Glycoside...

Embodiment 2

[0041] (1) Preparation of the test solution: dissolve the technical-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolve, then filter through a 0.45 μm filter membrane to obtain the test solution;

[0042] (2) liquid chromatographic preparation: inject the test solution into the preparation chromatographic separation pure fraction, wherein chromatographic conditions: chromatographic column is WatersXTerraRP18 (250mm * 20mm, 5 μ m), mobile phase: 25% (v / v) acetonitrile aqueous solution; Flow velocity : 10mL / min; column temperature: 25°C; running time: 15min; detection wavelength: 205nm, injection volume 0.1-1mL, collect fractions for 9-11min;

[0043] (3) The fractions were dried by placing them in a lyophilizer at -50°C for 24 hours. After the lyophilized mogroside V solids were ground and pulverized, they were lyophilized again for 24 hours to obtain mogroside V standard samples.

[0044] Store samples in a -18°C freezer.

[0045...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of analytical chemistry, and relates to a preparation method and application of a mogroside V standard sample. The preparation method provided by the invention comprises the following steps: 1) dissolving an industrial-grade siraitia grosvenorii crude extract in an organic solvent, performing dissolving with ultrasonic assistance, and performing filtering with a filter membrane to obtain a test solution; (2) separating the test solution obtained in the step (1) by using a high performance liquid chromatography, and collecting fractions after systematic elution for 9-11 minutes; and 3) fully freeze-drying and drying the fraction collected in the step 2) to obtain the mogroside V standard product. The preparation method disclosed by the invention is economical, rapid and effective, and can make up the technical blank in the aspect of development of the mogroside V standard sample in China.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a preparation method and application of a mogroside V standard sample. Background technique [0002] Mogroside V (Mogroside V) is the main source of the sweetness of Luo Han Guo. It has pharmacological effects such as cough and expectorant, anti-cancer, anti-oxidation, and blood sugar regulation. Its sweetness value at a concentration of 1 / 10,000 is 5% of sucrose 425 times. Therefore, it has become a new generation of functional sweeteners developed by domestic and foreign countries, and it is also a high-quality excipient in the pharmaceutical industry. [0003] However, there are many difficulties in the cultivation of Luo Han Guo, and the content of mogroside V in the whole fruit is only 0.8%-1.3% W / W, but one of the main indicators to evaluate the quality of Luo Han Guo is the high content of mogroside V . Therefore, in order to effectively screen the Luo Ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈巧谢晓烨王小宁宋云飞李杰
Owner SECOND MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products