Preparation method and application of mogroside V standard sample
A mogroside and standard sample technology, applied in the field of analytical chemistry, can solve the problems of complex purification difficulty of mogroside V analogs, inability to realize industrialized large-scale production, long delivery period, etc., and achieve stable product quality and preparation speed. Fast, high-volume results
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Embodiment 1
[0036] (1) Preparation of the test solution: dissolving the industrial-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolving it, and then filtering through a 0.22 μm filter membrane to obtain the test solution;
[0037] (2) Liquid chromatography preparation: inject the solution of the test sample into the preparation chromatography to separate pure fractions, wherein the chromatographic conditions: the chromatographic column is PREP-ODS(H) KIT (250mm×20mm, 5 μm), mobile phase: 23% (v / v) Acetonitrile aqueous solution; flow rate: 5-15mL / min; column temperature: 30°C; running time: 15min; detection wavelength: 202nm, injection volume 0.1-1mL, and collect fractions for 9-11min;
[0038] (3) The drying of the fractions is to freeze-dry in a freeze dryer at -60 to -40°C for 20 to 30 hours, and after the freeze-dried mogroside V solid is ground and pulverized, it is freeze-dried again for 20 to 30 hours to obtain Luo Han Guo Glycoside...
Embodiment 2
[0041] (1) Preparation of the test solution: dissolve the technical-grade Luo Han Guo crude extract (containing 80% mogroside V) in methanol and ultrasonically dissolve, then filter through a 0.45 μm filter membrane to obtain the test solution;
[0042] (2) liquid chromatographic preparation: inject the test solution into the preparation chromatographic separation pure fraction, wherein chromatographic conditions: chromatographic column is WatersXTerraRP18 (250mm * 20mm, 5 μ m), mobile phase: 25% (v / v) acetonitrile aqueous solution; Flow velocity : 10mL / min; column temperature: 25°C; running time: 15min; detection wavelength: 205nm, injection volume 0.1-1mL, collect fractions for 9-11min;
[0043] (3) The fractions were dried by placing them in a lyophilizer at -50°C for 24 hours. After the lyophilized mogroside V solids were ground and pulverized, they were lyophilized again for 24 hours to obtain mogroside V standard samples.
[0044] Store samples in a -18°C freezer.
[0045...
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