Method for grafting new variety of "Hongyu" camellia oleifera seedlings by using distant variety as stock

Abstract

The invention discloses a method for grafting a new variety of "Hongyu" camellia oleifera seedlings by using a distant variety as a stock. The method comprises the following steps of 1, identifying a genetic relationship; 2, stock selection; 3, vegetative propagation; 4, counting the survival rate; and 5, determination of stocks. In the first step, a SLAF-seq technology is adopted for performing simplified genome sequencing on the new variety of "Hongyu" camellia oleifera, Luchuan camellia oleifera, fragrant flower camellia oleifera, southern camellia oleifera, small-fruit camellia oleifera, common camellia oleifera, camellia chekiangoleosa and Bobai big-fruit camellia oleifera, polymorphic SLAF tags are developed, population SNP loci are obtained, and based on high-consistency population SNP, a neighbor-joining algorithm is adopted to carry out systematic genetic evolution relationship analysis on test germplasm; and the method is easy to operate, capable of obtaining the rootstock-scion combination with the high grafting survival rate, high in operability and beneficial to popularization, has practical significance in improving the scientific research and production efficiency, and can obtain good economic benefits, social benefits and ecological benefits to a certain extent.

Description

technical field

[0001] The invention relates to the technical field of plant cultivation, in particular to a method for grafting seedlings of a new variety of Camellia oleifera using distant species as stock. Background technique

[0002] The selection and breeding of Camellia oleifera varieties in my country began in the mid-1950s, and more than 20 excellent farm varieties and more than 6,000 excellent trees were screened out successively. 226 excellent clones, 19 excellent families, Five excellent hybrid combinations have laid a solid material foundation for the high-yield cultivation and improved varieties of camellia oleifera in my country. At present, the fine varieties of camellia oleifera in Guangxi are mainly ordinary camellia oleifera, and the variety structure is relatively single, which has greatly affected the healthy and sustainable development of the camellia oleifera industry. How to quickly create more and better new varieties and enrich the diversity of impr...

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