Application of glycosylglycerol in skin aging resistance

A glycerol glucoside, anti-aging technology, applied in the field of skin care, can solve the problems of complex preparation process and high addition amount of active ingredients, and achieve the effects of good safety, promoting proliferation and reducing impurity components

Pending Publication Date: 2021-06-11
青岛中科蓝智生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many anti-aging skin care products on the market, the main active substances include vitamin C, dipeptide, lycopene, vitamin E, polyphenols, etc.; however, the existing anti-aging products generally require a variety of active ingredients For compound use, the preparation process is relatively complicated, and the amount of active ingredients added is high, which will produce certain cytotoxicity

Method used

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  • Application of glycosylglycerol in skin aging resistance
  • Application of glycosylglycerol in skin aging resistance
  • Application of glycosylglycerol in skin aging resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1, the cultivation of high-content glycerol glucoside algae cells (GG), the method comprises the following steps:

[0046] (1) Obtain the cultured algae cell stage:

[0047]This algae cell is inoculated in the low-salt freshwater medium Zarrouk culture medium (see table 1 and table 2 for details), and initial inoculation concentration is 0.2g / L, and the temperature program of the freshwater medium of controlling cultivation this algae cell is: constant temperature 25°C, the light program is: continuous light, the light intensity is 500μE·m -2 ·s -2 , the light wavelength range is 400-700nm, the ventilation volume is: 0.3VVM, the gas passed through is mixed air containing carbon dioxide, and the carbon dioxide concentration is 5% (v / v).

[0048] The culture time at this stage is 5-7 days.

[0049] Wherein, the specific formula of the fresh water medium is Zarrouk medium (see Table 1 and Table 2 for details).

[0050] (2) GG generation stage:

[0051] The algae cells...

Embodiment 2

[0074] The glycerol glucoside prepared in Example 1 was carried out to the test of HaCaT cytotoxicity and growth-promoting effect:

[0075] 1. Instruments and materials

[0076] Human skin immortalized keratinocytes (HaCaT) were purchased from ATCC.

[0077] Primary reagent: PBS: 2164154

[0078] DMEM medium: 8120189

[0079] 0.25% Trypsin: 205359

[0080] Main instruments: CO2 incubator: 2015SW0058

[0081] Biological safety cabinet: 2015SW0026

[0082] Inverted microscope: 2015SW0056

[0083] Microplate reader: 2015SW0006

[0084] Water bath: 2015SW0023

[0085] Mixer: 2015SW0037

[0086] 2. Test samples

[0087] The sample is a water-soluble sample with a glycerol glucoside concentration of 50%. After being filtered through a 0.22 μm filter membrane, it is serially diluted with DMEM medium until the glycerol glucoside concentration is 10 times the concentration shown in Table 3.2.1 for later use.

[0088] Table 3 Sample concentration design table

[0089]

[0...

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Abstract

The invention belongs to the field of skin care, and particularly relates to application of glycosylglycerol in skin aging resistance. According to the characteristics of the glycosylglycerol low-permeability extract, the invention provides a set of glycosylglycerol purification method suitable for the extract. A stable and efficient glycosylglycerol purification technology is established by treating a specific glycosylglycerol hypotonic extract through a series of physical methods; chemical reaction is not needed; impurity components in a finished product are reduced; active components of glycosylglycerol are reserved to the maximum extent; and the purity of glycosylglycerol is up to 90% or above. The glycosylglycerol prepared by adopting the method has a more excellent skin anti-aging effect, can effectively promote proliferation of keratinocytes, realizes an excellent keratinocyte growth promoting effect when the volume concentration is only 0.01%, does not have obvious cytotoxicity, is better in safety, and the compound can be independently used as an active component to be widely applied to the skin anti-aging field.

Description

technical field [0001] The invention belongs to the field of skin care, and in particular relates to the application of glycerol glucoside in skin anti-aging. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Today's modern people are not only facing the pressure of life, but also the pressure of work is increasing. The skin is the largest organ covering the surface of the human body. Due to direct contact with the external environment and stimulation by various stress factors such as oxidation, dryness and ultraviolet rays, the skin is very vulnerable to damage. At the same time, due to the influence of internal factors such as age, diet and living habits, skin function d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61Q19/08A61Q19/00C07H15/04C07H1/06
CPCA61K8/602A61Q19/08A61Q19/00C07H15/04C07H1/06A61K2800/10
Inventor 张凯李新段仰凯吕雪峰
Owner 青岛中科蓝智生物科技发展有限公司
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