Preparation method and application of cichorium glandulosum extract lactucin

Abstract

The invention belongs to the field of biological medicine, and relates to a preparation method of cichorium glandulosum extract lactucin and anti-inflammatory and liver-protecting effects. The cichorium glandulosum guaiane type sesquiterpene lactone extract lactucin can be used as an inflammation lead compound, wherein the lactucin has an inhibition effect on NO, TNF-alpha and IL-6 generated by RAW264.7 cells activated by LPS, the cytotoxicity is low, the expression of iNOS and COX-2 proteins can be remarkably reduced, the expression of IL-6, iNOS, COX-2 and IFN-beta genes in the RAW264.7 cells can be remarkably inhibited, the P44/42 in an MAPK signal channel can be reduced, and the phosphorylation of P44/42 and P38 MAPK proteins in an MAPK signal channel can be reduced. Besides, the lactucin can inhibit activation of rat hepatic stellate cells stimulated by TGF-beta1, can inhibit Smad3, promote expression of Smad7 and TLR4 proteins and promote growth of bifidobacterium adolescentis to achieve the anti-hepatic fibrosis effect; furthermore, the compound can further reduce the concentration of IL-6 and IL-1beta in mouse serum caused by LPS and relieve mouse hepatic fibrosis tissue lesions caused by CCL4.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a preparation method and application of a chicory extract, in particular to a preparation method of guaiac-type sesquiterpene lactone extract lettucein and its application in anti-inflammatory and liver-protecting drugs role in. Background technique [0002] The liver is the largest organ in the human body and an important metabolic organ of the body. The liver has the functions of deoxidizing, storing sugar, secreting and synthesizing protein in the body, and also secretes bile needed by the digestive system. Liver diseases include viral hepatitis, non-viral hepatitis, liver cancer, liver fibrosis, etc. Liver disease has become one of the diseases that seriously threaten people's quality of life and life expectancy. The multidisciplinary comprehensive treatment model involving traditional Chinese medicine is a major strategy for the prevention and treatment of liver diseases in my count...

AI Technical Summary

Problems solved by technology

[0003] At present, the anti-hepatitis drugs are mainly western medicines, and the application of traditional Chinese medicines is relatively seldom. What is worrying is that western medicines often cause serious toxic and side effects, and the research and development costs are relatively high, while traditional Chinese medicines cost less than western medicines, and have fewer adverse drug reactions. High safety and few side effects

However, the hepatoprotective and antibacterial effect of the original medicinal material Chicory is not obvious, and it is necessary to separate and purify components with anti-inflammatory and liver-protecting effects from Chicory in order to better exert the anti-inflammatory and liver-protecting effects of Chicory

The research on the liver protection of chicory mainly focuses on the anti-inflammatory effect of chicory water extract (experimental research on the anti-inflammatory effect of water extract of chicory hair, a Uyghur drug, Yang Qiaoli, China Medical Herald, 2016) and the effect of chicory extract on liver fibrosis. function, so as to prevent various liver diseases caused by inflammation, and play a certain role in liver protection. This kind of research reveals the molecular mechanism of the water extract of hairy chicory to inhibit inflammation, but there are still many problems. It is difficult to study the pharmacology and toxicology of the mixture as a single compound of inflammatory effect, thus limiting the clinical application of the water extract of chicory hair. In addition, chicory hair also contains a large amount of water-insoluble components, which are harmful to the original The anti-inflammatory effect of Chicory pilosula also has a certain contribution, but the scheme of the prior art lacks the research on the anti-inflammatory effect of non-water-soluble components

Moreover, the existing research failed to further explore the role of chicory in protecting the liver from the perspective of the inflammatory microenvironment leading to the deterioration of liver disease. In addition, studies have shown that the immune inflammatory response caused by intestinal microorganisms can always run through the development of liver fibrosis , but at present, there are few related studies on reducing intestinal inflammation and improving intestinal flora to protect the liver and resist liver fibrosis. Therefore, from the perspective of anti-inflammatory, improving the inflammatory microenvironment, and improving intestinal flora to protect the liver It will be of great significance to explore the application of the traditional Chinese medicine chicory monomer compound in the prevention and treatment of liver disease and liver protection

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