A kind of methylotrophic bacillus bam-6 and its application
A methylotrophic, BAM-6 technology, applied in applications, bacteria, fungicides, etc., to achieve the effects of promoting plant growth, increasing yield, and strong stress resistance
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Embodiment 1
[0023] Example 1 Isolation and Screening of Methylotrophic Bacillus BAM-6
[0024] (1) Take soil samples: choose a well-growing potato plot (Guizhou), take the rhizosphere of healthy potato plants, shovel off 2-3 cm of the topsoil, and take 20-50 g of potato rhizosphere soil; Seal the mouth of the bag, and record the sampling location, environment and date.
[0025] (2) Isolation and purification of strains: The soil samples were isolated by plate dilution method on PB medium. The specific operation is as follows: Accurately weigh 10 g of soil sample, add it into a 90 mL conical flask of 0.9% normal saline with glass beads, shake it on a shaker at 180 r / min for 30 min, and make the soil sample evenly distributed to make a soil suspension. for 10 -1 ; diluted sequentially to a concentration of 10 -5 、10 -6 、10 -7 . They were placed in a constant temperature water bath at 80 °C for 30 min, and 100 µL of the dilution was taken to coat PB plates, and the plates were incubate...
Embodiment 2
[0031] Example 2 Identification of Methylotrophic Bacillus BAM-6
[0032] Morphological characteristics and physiological and biochemical experiments: According to the experimental methods in the "Common Bacterial System Identification Manual", the screened strain BAM-6 was subjected to morphological, physiological and biochemical identification. Such as figure 1 As shown, the single colony formed by this strain on the PB medium plate is milky white, opaque, nearly round, with neat edges in the early stage and irregular folds in the later stage. It is dry and easy to pick. After 48 hours of culture, the colonies were picked and cultured for Gram staining, and the Gram staining was positive; under the observation of an optical microscope with 100 times oil lens, the strain was bacillus, which produced spores, and the spores were oval in shape. Its physiological and biochemical identification indicators are shown in Table 1.
[0033] Table 1 Physiological and biochemical char...
Embodiment 3
[0038]Example 3 Determination of the Bacterial Inhibition Spectrum of Methylotrophic Bacillus BAM-6
[0039] Select common plant fungal disease pathogens such as: potato dry rot, potato early blight, potato fusarium wilt, potato ring rot, pear black spot, tomato gray mold, pepper anthracnose, tomato root rot, cucumber gray mold, Cotton wilt, grape white rot, grape cinerea, apple ringworm, tobacco Alternaria and other plant pathogenic fungi were used as targets, and the antibacterial spectrum of strain BAM-6 was determined by plate confrontation method. The strain BAM-6 has a strong antagonistic effect on the 14 kinds of plant fungal diseases tested, among which the inhibitory effect on potato dry rot is the strongest, and the diameter of the inhibition zone can reach 30.15 mm, followed by Fusarium wilt, and the inhibition zone reaches 29.28mm.
[0040] Table 2 Inhibitory effects of methylotrophic Bacillus BAM-6 on different plant pathogenic fungi
[0041] .
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