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Hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as preparation method and application of hernia repair material

A technology of gelatin sponge and stem cells, which is applied in the field of bioengineering, can solve the problems of poor strengthening strength, low survival rate of stem cells, and high cost, and achieve the effects of strengthening strength, improving survival rate, and delaying degradation speed

Inactive Publication Date: 2021-06-15
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current technology cannot simultaneously overcome the shortcomings of insufficient strength and rapid absorption of biological mesh when repairing hernia defects, and the cost is relatively high
[0004] In the prior art, there are also studies on the combination of stem cells and biological patches for hernia repair, but there are still problems such as low survival rate of stem cells and poor reinforcement strength

Method used

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  • Hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as preparation method and application of hernia repair material
  • Hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as preparation method and application of hernia repair material
  • Hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as preparation method and application of hernia repair material

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preparation example Construction

[0035] In one embodiment of the present invention, a method for preparing a hernia repair material composed of the above-mentioned composite adipose-derived mesenchymal stem cells and gelatin sponge is provided, comprising the following steps:

[0036] Firstly, adipose-derived mesenchymal stem cells were isolated and cultured from adipose tissue; then the cultured adipose-derived mesenchymal stem cells were filled into gelatin sponge; the gelatin sponge filled with adipose-derived mesenchymal stem cells was spread under the biological patch, and the obtained A hernia repair material composed of adipose-derived mesenchymal stem cells and gelatin sponge;

[0037] In one or more embodiments, the adipose-derived mesenchymal stem cells are cultured to the fourth generation;

[0038] In one or more embodiments, the gelatin sponge filled with adipose-derived mesenchymal stem cells and the biological patch can also be placed separately, and the biological patch is covered on top of th...

Embodiment 1

[0044] 1.1. Isolation and identification of rat adipose-derived mesenchymal stem cells (rADMSCs)

[0045] 1.1.1. Isolation and culture

[0046] Observed under an inverted microscope, after subculture, the cell shape gradually became uniform, similar to fibroblasts, mostly in the shape of a long spindle or spindle, with irregular shapes and triangles in between, such as figure 1 shown.

[0047] 1.1.2. Identification

[0048] (1) CD29 and CD90 are highly expressed in the 4th generation ADMSCs of SD rats, and CD34 and CD45 are negatively expressed, see figure 2 .

[0049] (2) Identification of rADMSCs differentiation potential

[0050] ① Identification of adipogenic differentiation potential

[0051] The 4th generation rADMSCs were taken, and induced and differentiated with SD rat adipose-derived mesenchymal stem cell adipogenic differentiation complete medium, and stained with Oil Red O staining solution on the 17th day, and the oil red O staining showed that the adipogenic ...

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Abstract

The invention relates to the technical field of bioengineering, in particular to a hernia repair material compounded with adipose-derived mesenchymal stem cells and gelatin sponge as well as a preparation method and application of the hernia repair material. The material comprises a biological patch and gelatin sponge on the lower side of the biological patch, and the gelatin sponge is filled with adipose-derived mesenchymal stem cells; the biological patch is of a natural fiber structure mainly composed of type I and type III collagen, and the number of adipose-derived mesenchymal stem cells in the gelatin sponge is 1 * 10 < 6 > to 3 * 10 < 6 > / cm < 2 >. After the gelatin sponge filled with the adipose-derived mesenchymal stem cells is compounded with the biological patch, the survival rate of the adipose-derived mesenchymal stem cells is increased, the degradation speed of the biological patch is delayed, wound healing is accelerated, muscle-like tissues are formed in vivo, the strength of the biological patch is enhanced, and the purpose of better treating abdominal wall soft tissue defect (hernia) is achieved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a hernia repair material composed of adipose-derived mesenchymal stem cells and gelatin sponge, a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The main component of biological mesh is collagen, which has become a hot spot in the research of hernia and abdominal wall surgery because of its good tissue compatibility, anti-infection, anti-adhesion, and complete degradation in vivo. Abdominal wall swelling and postoperative recurrence rate after application of bio-mesh are high, which is related to insufficient strength and rap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/38A61L27/56A61L27/58
CPCA61L27/222A61L27/3834A61L27/56A61L27/58A61L2430/34C08L89/00
Inventor 李波刘俏男栾立江淼张光永刘晓丽
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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