Ionizable lipid molecule, preparation method thereof, and application of ionizable lipid molecule in preparation of lipid nanoparticles

A lipid nanoparticle and molecular technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of not being able to fully exert the efficacy of mRNA-LNP preparations, achieve excellent mRNA carrier performance, and improve the effect of translation and expression levels

Active Publication Date: 2021-06-15
IMMORNA (HANGZHOU) BIOTECHNOLOGY CO LTD
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing LNP component scheme cannot fully exert the effectiveness of the mRNA-LNP preparation, and it is necessary to continuously adjust the structure of ionizable lipid molecules and optimize the design of specific RNA. The present invention solves such problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ionizable lipid molecule, preparation method thereof, and application of ionizable lipid molecule in preparation of lipid nanoparticles
  • Ionizable lipid molecule, preparation method thereof, and application of ionizable lipid molecule in preparation of lipid nanoparticles
  • Ionizable lipid molecule, preparation method thereof, and application of ionizable lipid molecule in preparation of lipid nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0033] An ionizable lipid molecule with a chemical structural formula: (Code: XH-04).

[0034] The following abbreviations represent the following reagents respectively:

[0035] DCM: dichloromethane; DMAP: 4-dimethylaminopyridine; TEA: triethanolamine, EDCI: 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride; TLC: silica gel plates Thin layer chromatography; THF: tetrahydrofuran; DMF dimethylformamide; DSPC: 1,2-distearate-sn-glycerophosphatidylcholine, DOPE: 1,2-dioleoyl-sn-glycerol-3- Phosphoethanolamine, TFF: Tangential Flow Filtration.

[0036] A preparation method of ionizable lipid molecules such as figure 1 Shown; specifically include the following steps:

[0037] Step 1, compound 1 in DCM dichloromethane (100mL) (20.0g, 89.6mmol, 1.00 equiv) was added to the solution of compound 1A (14.2 g, 98.6 mmol, 1.10 equiv)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ionizable lipid molecule, a preparation method thereof, and application of the ionizable lipid molecule in the preparation of lipid nanoparticles. The ionizable lipid molecule has a chemical structure shown in the description with the code name being XH-04; the novel ionizable lipid molecule XH-04, phospholipid, cholesterol and polyethylene glycol are subjected to microfluidic synthesis to obtain the LNP, the obtained LNP can improve the translation expression level of a load, namely mRNA, in cells, the action efficacy of an mRNA-LNP preparation is improved, and a theoretical basis is provided for theoretical treatment of a personalized mRNA-LNP preparation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an ionizable lipid molecule and its preparation method and its application in the preparation of lipid nanoparticles. Background technique [0002] Nucleic acids (RNA) have great therapeutic potential in modulating protein expression in vivo, but naked RNA is internalized by cells with extremely low efficiency, and it is easily cleared by the kidney during in vivo delivery, or quickly by RNases in vivo. Degradation, its actual curative effect is greatly reduced. In order to exert the therapeutic ability of RNA more safely and effectively, scientists use lipid nanoparticles (LNP) to encapsulate and deliver RNA to specific parts of the body. This RNA delivery strategy has shown great utility in the delivery of double-stranded small interfering RNAs (siRNAs, 21 to 23 nucleotides in length). For example, lipidoid C12-200 has been widely used to make siRNA-LNP preparations for various t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C229/12C07C227/18A61K9/51A61K47/18A61K31/7088B82Y5/00B82Y40/00
CPCC07C229/12C07C227/18C07C67/31C07C67/08A61K9/5123A61K31/7088B82Y5/00B82Y40/00C07C69/712C07C69/63
Inventor 王子豪
Owner IMMORNA (HANGZHOU) BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap