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Cell or stem cell cryopreservation preparation method

A stem cell and cell technology, applied in the medical field, can solve the problems of bulky refrigerators and storage equipment, which cannot meet clinical applications, and expensive storage costs, and achieve the effects of convenient infusion, improved clinical use, and long storage time

Pending Publication Date: 2021-06-18
陕西光子动力航天科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Both of these two preservation methods require bulky refrigerators and storage equipment, are expensive to preserve, and are limited by many natural and environmental factors during preservation and transportation, which are far from meeting clinical applications.

Method used

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  • Cell or stem cell cryopreservation preparation method

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Embodiment Construction

[0020] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0021] according to figure 1 , a cell or stem cell cryopreservation preparation method, which mainly includes the following steps:

[0022] Step 1, protecting the stem cells with a protective agent;

[0023] Step 2, pre-freezing the stem cells;

[0024] Step 3, performing primary drying on the pre-frozen stem cells;

[0025] Step 4, performing secondary drying on the stem cells after the primary drying.

[0026] The cell products freeze-dried in the above steps can be stored at room te...

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Abstract

The invention relates to the technical field of medicine, in particular to a cell or stem cell cryopreservation preparation method which comprises the following steps: step 1, protecting stem cells through a protective agent; step 2, pre-freezing the stem cells; step 3, carrying out primary drying on the pre-frozen stem cells; and 4, carrying out secondary drying on the stem cells subjected to primary drying. The freeze-dried cells can be preserved at normal temperature through the protective agent, the performance is stable, the preservation time is long, transportation is convenient, the preservation cost is low, and the limitation of current cell preservation is solved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a preparation method for cryopreservation of cells or stem cells. Background technique [0002] At present, short-term storage at room temperature and long-term storage at deep low temperature are mostly used for storage of cells and stem cells. Both of these two preservation methods require bulky refrigerators and storage equipment, are expensive to preserve, and are limited by many natural and environmental factors during preservation and transportation, which are far from satisfying clinical applications. [0003] Because freeze-dried cells can be stored at room temperature, have a long storage time, relatively stable performance, convenient infusion, low storage costs, and convenient transportation, the freeze-dried storage of cells has become a research hotspot. The successful development of freeze-dried cells and stem cells will improve the clinical situation and have gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 王之洞
Owner 陕西光子动力航天科技有限公司
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