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Lentinan nano-selenium, preparation method and application of lentinan nano-selenium in treating malignant pleural effusion

A technology of lentinan and nano-selenium, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., to achieve the effects of improving bioavailability, good bioresponse performance, biocompatibility, and good biosafety

Pending Publication Date: 2021-06-22
GUANGDONG JINAN ESTABLISHED SELENIUM SOURCE NANO TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing polysaccharide and selenium nano-medicines have little effect on malignant pleural effusion, especially the malignant pleural effusion caused by lung adenocarcinoma. Therefore, it is of great significance to develop a class of nano-medicines that can be used to treat malignant pleural effusion

Method used

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  • Lentinan nano-selenium, preparation method and application of lentinan nano-selenium in treating malignant pleural effusion
  • Lentinan nano-selenium, preparation method and application of lentinan nano-selenium in treating malignant pleural effusion
  • Lentinan nano-selenium, preparation method and application of lentinan nano-selenium in treating malignant pleural effusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Diagnosis and clinical identification of patients with malignant pleural effusion (MPE-LA) associated with lung adenocarcinoma

[0055] High-resolution chest CT scans were performed on 20 patients with lung adenocarcinoma-associated malignant pleural effusion, all with MPE or pleuropulmonary accumulation. Before pleural biopsy, pleural effusions were collected from all subjects for biochemical analysis. Then, pleural biopsy was performed using ultrasound-guided cutting-needle biopsy combined with conventional pleural biopsy. Collect more than 50 mL of pleural effusion for cytological examination (H&E staining; NapsinA expression and PD-L1 were detected by immunohistochemistry; lactate dehydrogenase (LDH) and carcinoembryonic antigen (CEA) were detected by corresponding commercially available assays detected by the kit).

[0056] Diagnosis results (since the test results of the 20 patients were similar, only the test results of one of the patients are shown h...

Embodiment 2

[0057] Example 2 Detection of selenium content in the blood and effusion of patients with lung adenocarcinoma-related malignant pleural effusion

[0058]Collect blood from patients with malignant pleural effusion associated with lung adenocarcinoma and centrifuge at 3000 rpm for 5 minutes at 4°C, take the supernatant, and use nitric acid and perchloric acid with a volume ratio of 3:1 (nitric acid: 16mol / L, perchloric acid: 12.38mol / L) were digested at 180°C, and the effusion samples from patients with malignant pleural effusion related to lung adenocarcinoma were digested by the same method, and the selenium content was detected by atomic fluorescence spectroscopy. Blood samples from healthy individuals were used as controls.

[0059] Test results such as figure 2 The results show that the blood selenium content of healthy people is about 232.6 μg / L, while the blood selenium content of lung adenocarcinoma-related MPE patients is about 139.6 μg / L. It can be seen that MPE pat...

Embodiment 3

[0060] Example 3 Preparation, Morphology and Structural Characterization of Lentinan Nano Selenium

[0061] (1) ①Under sterile conditions, dissolve 75mg of lentinan (LNT) powder (Shaanxi Senfu Biotechnology Co., Ltd.) Ultrasonic under conditions to make it fully dissolved (time 2min); filter once with a 0.45 μm sterile filter; then filter three times with a 0.22 μm sterile filter to obtain a lentinan solution;

[0062] ②Under sterile conditions, 0.0176g sodium selenite (Na 2 SO 3 ) powder was dissolved in 10mL sterile ultrapure water and filtered with a 0.22 μm sterile filter to obtain a sodium selenite solution;

[0063] ③Under sterile conditions, dissolve 0.0704g of ascorbic acid (Vc) powder in 10mL of sterile ultrapure water and filter with a 0.22 μm sterile filter membrane to obtain an ascorbic acid solution;

[0064] ④Under sterile conditions, take 1mL lentinan solution and mix them with 2mL sodium selenite solution and 2mL ascorbic acid solution respectively to obtain...

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Abstract

The invention discloses lentinan nano-selenium, a preparation method and application of the lentinan nano-selenium in treatment of malignant pleural effusion. The preparation method comprises the following steps: (1) respectively dissolving lentinan, sodium selenite and ascorbic acid in water to obtain corresponding solutions; (2) uniformly mixing the lentinan solution with a sodium selenite solution and an ascorbic acid solution respectively to obtain a lentinan / sodium selenite mixed solution and a lentinan / ascorbic acid mixed solution; and (3) dropwise adding the lentinan / sodium selenite mixed solution into the lentinan / ascorbic acid mixed solution, and conducting stirring for reaction to obtain the lentinan nano-selenium. Through comparison, it is found that the lentinan nano-selenium prepared through the method can be taken by immune cells in malignant pleural effusion (MPE), MPE in a cold state is converted into functional hot state, the immune function of a patient is improved, and therefore the lentinan nano-selenium can be used for treating tumors and malignant pleural effusion.

Description

technical field [0001] The invention relates to the technical field of treatment of malignant pleural effusion, in particular to a lentinan nano-selenium and its preparation method and its application in the treatment of malignant pleural effusion. Background technique [0002] The 2018 global cancer statistics show that lung cancer remains the leading cause of cancer incidence (11.6%) and mortality (18.6%) worldwide. Of the 2.1 million newly diagnosed cases of lung cancer, approximately 85% are non-small cell lung cancer (NSCLC). Malignant pleural effusion (MPE), characterized by the presence of malignant cells or metastases in the pleura, occurs in about 40% of patients with advanced non-small cell lung cancer (NSCLC), and is more common in lung adenocarcinoma (LA). [0003] Although substantial progress has been made in the field of anti-tumor therapy, LA-induced MPE (MPE-LA) still faces problems such as poor prognosis, short life expectancy (3-12 months), and low respon...

Claims

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Application Information

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IPC IPC(8): A61K33/04A61K31/715A61K9/14A61P7/10A61P35/00A61P37/04B82Y5/00
CPCA61K33/04A61K31/715A61K9/14A61P7/10A61P35/00A61P37/04B82Y5/00
Inventor 陈填烽宋桭环汪金林黄狄娜李海伟陈义康
Owner GUANGDONG JINAN ESTABLISHED SELENIUM SOURCE NANO TECH RES INST CO LTD
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