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Bifidobacterium bifidum for relieving diarrhea colon and application of bifidobacterium bifidum

A technology of bifidobacteria bifidum and colon, applied in the field of microorganisms, can solve the problems of different effects of constipation, and achieve the effects of shortening the time of the first black stool, excellent growth characteristics, and increasing the content of butyric acid

Active Publication Date: 2021-06-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As an effective adjuvant treatment to relieve constipation, probiotics have a good laxative effect, but due to various causes of constipation, probiotics have different effects on different types of constipation
However, in the current study, probiotics have not been included in the treatment of laxative colon

Method used

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  • Bifidobacterium bifidum for relieving diarrhea colon and application of bifidobacterium bifidum
  • Bifidobacterium bifidum for relieving diarrhea colon and application of bifidobacterium bifidum
  • Bifidobacterium bifidum for relieving diarrhea colon and application of bifidobacterium bifidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The screening, identification and cultivation of embodiment 1 Bifidobacterium bifidum CCFM1163

[0043] (1) Isolation and screening of bacterial strains:

[0044] (1) Use a disposable sterile toilet to collect the feces of adult men in Yongchang, Gansu Province, spread the feces samples in the medium of MRS+0.08% cysteine ​​by mass percentage, and place them in an anaerobic incubator (N 2 :CO 2 :H 2 =80:10:10) enriched for 12h;

[0045] (2) Stool samples were serially diluted with sterile normal saline, and then spread on MRS + 0.08% by mass percentage of L-cysteine ​​added with sterile 100 μg / mL mupirocin and 50 U / mL nystatin acid salt solid plate, cultured for 24-48h;

[0046] (3) Select a single colony conforming to the basic form of bifidobacteria to carry out streaking and purification on the plate, and screen and isolate the selected bacterial strain;

[0047] (4) The above-mentioned single colonies were cultured in liquid MRS+0.08% by mass cysteine ​​culture...

Embodiment 2

[0074] Example 2: Relief effect of Bifidobacterium bifidum CCFM1163 on senna laxative colonic mice

[0075] Take out Bifidobacterium bifidum CCFM1163 from the -80°C refrigerator, streak it on the MRS plate, culture at 37°C for 24 hours, pick a single colony in the MRS liquid tube, and culture it at 37°C for 20 hours. Inoculate in new MRS liquid medium, cultivate at 37°C for 16h, and culture for another generation in the same way, then centrifuge the Bifidobacterium bifidum suspension at 6000r / min, 4°C for 5min, and then use 10% ( m / v) skimmed milk (prepared by dissolving 10g skimmed milk powder in 100mL water) was resuspended to obtain a bacterial concentration of 5×10 9 CFU / mL bacterial suspension for animal experiments.

[0076] Twenty-four healthy male C57BL / 6J mice aged 7 weeks were taken, fed for 1 week to adapt to the environment, and randomly divided into 4 groups: control group (normal), model group (model), mosapride group (mosapride) and dichotomy group. Bifidobact...

Embodiment 3

[0086] Example 3: Bifidobacterium bifidum CCFM1163 increases the number of glial cells in the colon tissue of cathartic colonic mice

[0087] C57BL / 6J mice grouping, modeling and treatment methods were the same as in Example 2. The number of glial cells in enteric nerves was quantified by immunofluorescent labeling of the glial cell-characteristic protein S100 beta. The specific method is as follows:

[0088] When the mice were sacrificed, fresh colon tissues were removed and rinsed with normal saline, immediately put into neutral buffered paraformaldehyde fixative solution for 24 hours, and rinsed with running water overnight. 30min, then add 95%, 100% each twice, 20min each time. The sample was passed through 1 / 2 pure alcohol, 1 / 2 xylene equal volume mixture for 15 minutes, xylene I for 15 minutes, and II for 15 minutes until transparent, then put in the mixed solution of xylene and paraffin for 15 minutes, then put in paraffin I and paraffin II to see through. Wax for 50...

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Abstract

The invention discloses bifidobacterium bifidum for relieving diarrhea colon and application of the bifidobacterium bifidum, and belongs to the technical field of microorganisms. The bifidobacterium bifidum CCFM1163 provided by the invention can shorten the first black stool time, increase the water content of feces, increase the number of glial cells in colon tissues, increase the expression quantity of glial cell-derived neurotrophic factors GDNF in colon, increase the content of acetylcholin esterase AChE in colon, increase the content of butyric acid in feces, increase the number of Cajal interstitial cells in colon, reduce the content of gastrointestinal hormone VIP in the colon, reduce the expression quantity of aquaporin AQP4 and AQP8 in the colon, and improve the health state of the intestinal tract, thereby alleviating purgative colon fundamentally. The bifidobacterium bifidum can be applied to functional foods and medicines for relieving diarrhea colon, and has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of bifidobacterium bifidum for relieving laxative colon and application thereof, belonging to the technical field of microbes. Background technique [0002] Laxative colon is a condition in which the long-term use of laxatives damages the colonic nervous system, leading to degenerative changes in enteric nerves, gastrointestinal motility disorders, and decreased responsiveness to laxatives, making patients dependent on laxatives. Middle-aged and elderly people suffering from constipation. [0003] In my country, the incidence of chronic constipation is as high as 20%. A series of stimulant laxatives such as senna, rhubarb, and aloe are favored by patients with constipation due to their quick effect and low cost. Patients need to use laxatives for a long time due to constipation. The dose of laxatives is inversely proportional to the effect, and finally loses the cathartic effect, forming a "laxative colon". In severe c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L19/00A23L19/10A23L11/50A23L11/65A23L11/45A23C9/123A23C19/00A23C13/16A23G3/36A61K35/745A61P1/00A61P1/10C12R1/01
CPCC12N1/20A23L33/135A23L29/065A23L19/00A23C9/1234A23C19/00A23C13/16A23C11/106A23C20/025A23L19/10A23G3/366A61K35/745A61P1/00A61P1/10A23V2002/00A23V2400/517A23V2200/3204
Inventor 王刚李鑫萍王琳琳赵建新陈卫张灏
Owner JIANGNAN UNIV
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