Microscope focus locking system
A microscope and microscope objective lens technology, applied in the microscope field, can solve the problem that the focus locking system cannot achieve sensitivity
Pending Publication Date: 2021-06-25
广东粤港澳大湾区黄埔材料研究院
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[0004] However, the existing focus locking system uses the lateral magnification of the optical system, and the displacement of the signal on the detector must be at least greater than one pixel before it can be perceived. The focus locking system based on this principle cannot achieve high sensitivity
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[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0022] Such as figure 1 As shown, an embodiment of the present invention provides a microscope focus locking system, including: a detection light source 10, a half mirror 11, a dichroic mirror 12, a microscope tube lens 13, a microscope objective lens 14, a first lens 16, beam splitter 17, first slit element 181, second slit element 182, first avalanche diode 191 and second avalanche diode 192;
[0023] The first slit element 181 is connected to the first avalanch...
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Abstract
The invention discloses a microscope focus locking system which comprises a detection light source, a semi-transparent and semi-reflecting mirror, a dichroscope, a microscope tube lens, a microscope objective lens, a first lens, a beam splitter, a first slit element, a second slit element, a first avalanche diode and a second avalanche diode. An incident light beam sequentially passes through the semi-transparent and semi-reflective mirror, the dichroscope, the microscope tube lens and the microscope objective lens and then enters a glass slide of a sample. The reflected light beam sequentially passes through a microscope objective lens, a microscope tube lens, a dichroscope, a semi-transparent and semi-reflective mirror and a first lens and then enters a beam splitter. The beam splitter divides the reflected light beam into a first light beam and a second light beam which are respectively emitted into a first avalanche diode and a second avalanche diode. The beam splitter is located between the light beam convergence focus and the first lens, and the sum of the distance from the first avalanche diode to the beam splitter and the distance from the second avalanche diode to the light beam convergence focus is equal to the distance from the beam splitter to the light beam convergence focus. According to the invention, the focus locking precision can be improved.
Description
technical field [0001] The invention relates to the technical field of microscopes, in particular to a microscope focus locking system. Background technique [0002] In the process of microscopic imaging, due to the vibration interference of the external environment, the position of the sample will deviate in the direction of the optical axis, resulting in blurred image plane, especially in the super-resolution imaging system based on single molecule positioning, the positioning deviation of a single point will make the heavy The resolution of the framed image is reduced. [0003] Based on the necessity of focusing plane locking in microscopic imaging, many companies have developed automatic focus locking systems. The existing focus locking system adopts the principle of lateral magnification, which converts the axial deviation of the sample into lateral deviation, and then amplifies through the optical system, and the CCD camera detects the feedback signal, and then moves ...
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IPC IPC(8): G01N21/84G01N21/01G02B21/24G01B7/312
CPCG01B7/312G01N21/01G01N21/84G02B21/241
Inventor 孙佳音王宏达赵天野
Owner 广东粤港澳大湾区黄埔材料研究院