Skin rejuvenation protein marker-ABRX2 protein and non-invasive extraction method thereof
A skin and skin aging technology, applied in the field of molecular biology, can solve the problem of judging the degree of skin aging without ABRX2 protein, and achieve the effect of simple method, accurate result and high efficiency
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[0056] Taking white-collar workers with a relatively close living environment and healthy body as samples, the epidermis skin samples were sampled through a non-invasive method (specifically, 3M adhesive skin, and the sampling was completed).
[0057] The present invention is to the measuring method of protein content in the experimenter's epidermis skin sample, according to the following steps:
[0058] (1) HPLC
[0059] Sampling of epidermis skin samples of subjects: stick 3M glue (the manufacturer of 3M medical stickers is 3M Health Care 3MBrookings) on the curved side of the forearm to eliminate photoaging factors; after 1 minute, gently peel off the 3M stickers , to ensure that the subject's skin was not damaged, and obtain a tape-like skin sample.
[0060] Obtaining of dried peptide samples: 1) Tape-like skin samples were cut into small pieces (0.5 x 0.5 cm) with a sterile razor blade and deposited on glass plates before transferring to 1.5 ml centrifuge tubes;
[0061...
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