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Method for rapidly extracting microalgae protein

A technology of microalgae and protein, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of microalgae protein volatilization, low protein content, inconvenient use, etc., and achieve low cost and shorten operation time, ease of use

Inactive Publication Date: 2021-06-29
YUNNAN AIERKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the methods used by microalgae to extract protein are usually time-consuming, which makes the cost of microalgae protein extraction relatively high. Inconvenience, for this reason, we propose a method for rapid extraction of microalgae protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] During the extraction operation, the microalgae are pretreated first, and the picked microalgae are washed in turn. When cleaning, the algae are first rinsed in an alkaline water pool for 10 minutes, and then the algae are placed in a clear water pool for 3 times. The drying time is 5 minutes; the drying temperature is set to 25°C, the baking time is 3 minutes, ground and pulverized, dried, and sieved to obtain the microalgae powder; then stirred, the obtained microalgae powder is placed in the mixer, and then added 8 times of buffer solution was stirred evenly. The buffer solution was Tris buffer solution, its pH value was 6.5, and it was allowed to stand for 60 minutes. Then, compound enzyme and organic solvent were added to stir again. The organic solvent was ethanol, and its concentration was 40%, the stirring speed is 50-80r / min, the compound enzyme is a proteolytic enzyme, after stirring evenly, a mixed solution is obtained; secondly, the extracted mixed solution i...

Embodiment 2

[0023] During the extraction operation, the microalgae are firstly pretreated, and the picked microalgae are washed in sequence. When cleaning, the algae are first rinsed in an alkaline water pool for 20 minutes, and then the algae are placed in a clear water pool for 5 times. 5min; when drying, the temperature is set to 40°C, and the baking time is 5min, and the microalgae powder is obtained by grinding, pulverizing, drying, and sieving; after stirring, the obtained microalgae powder is placed in a blender, and then added 10 times the buffer solution is stirred evenly, the buffer solution is Tris buffer solution, its pH value is 7, let it stand for 70min, then add compound enzyme and organic solvent to stir again, the organic solvent is styrene, its concentration is 45%, the stirring speed is 80r / min, the complex enzyme is carboxylase, after stirring evenly, a mixed solution is obtained; secondly, after extraction, the obtained mixed solution is left to stand for 30 minutes, h...

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Abstract

The invention discloses a method for rapidly extracting microalgae protein. The method comprises the following steps: firstly, pretreating microalgae, specifically, sequentially cleaning, grinding, drying and sieving picked microalgae to obtain microalgae powder; and secondly, conducting stirring treatment, specifically, putting the obtained microalgae powder into a stirrer, then adding 8-10 times of a buffer solution, uniformly stirring, standing for 60-120 min, then adding a compound enzyme and an organic solvent, and stirring again at the stirring speed of 50-80 r / min to obtain a mixed solution after uniformly stirring. According to the method for rapidly extracting the microalgae protein, the buffer solution and the organic solvent are added in the preparation process, so that the mixed solution can rapidly separate and gather the protein in microalgae during extraction, preparation conditions are provided for subsequent rapid protein extraction, the method is short in time consumption, the whole extraction process can be completed in only about 5 hours, the operation time can be effectively shortened on the basis of ensuring the extraction rate, and the method has a good use prospect.

Description

technical field [0001] The invention belongs to the technical field of microalgae extraction, in particular to a method for rapidly extracting microalgae protein. Background technique [0002] Protein is the material basis of all life and an important part of cells. Life activities such as body growth, development, movement and reproduction are inseparable from protein. According to surveys, the growth rate of population has far exceeded the growth rate of food protein supply , while microalgae are rich in protein, with high nutritional value, and the intracellular protein content can reach 1.25-1.5 times that of soybean; [0003] At present, the methods used by microalgae to extract protein are usually time-consuming, which makes the cost of microalgae protein extraction relatively high. Inconvenience, for this reason, we propose a method for rapid extraction of microalgae protein. Contents of the invention [0004] The main purpose of the present invention is to provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
CPCC07K1/145C12P21/06
Inventor 张勇李林品吴秋瑾
Owner YUNNAN AIERKANG BIOTECH
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