A method for screening pivotal genes and key signaling pathways of perfluorooctane sulfonate toxicity using transcriptomics

A gene and pathway technology, applied in the field of transcriptomics screening of PFOS toxicity hub genes and key signaling pathways

Active Publication Date: 2022-01-04
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that PFOS exhibits various toxic effects on animals such as hepatotoxicity, testicular toxicity, pancreatic toxicity, neurotoxicity, immunotoxicity, reproductive and developmental toxicity, and induces the occurrence of related tumors.

Method used

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  • A method for screening pivotal genes and key signaling pathways of perfluorooctane sulfonate toxicity using transcriptomics
  • A method for screening pivotal genes and key signaling pathways of perfluorooctane sulfonate toxicity using transcriptomics
  • A method for screening pivotal genes and key signaling pathways of perfluorooctane sulfonate toxicity using transcriptomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. The establishment and result analysis of the method for evaluating the toxicity mechanism of PFOS using transcriptomics

[0044] 1. Acquisition of transcriptomics data

[0045] 1.1 PFOS treatment

[0046] The experiments described below consisted of three replicates.

[0047] (1) Select 6-8 week-old Sprague-Dawley rats (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), 8 in total, 4 males and 4 males. The 8 rats had the same or similar date of birth and similar body weight among the same sex. The age (6 weeks for male rats and 6 weeks for female rats) and body weight (281±13g for male rats and 190±6g for female rats) were recorded. Eight rats were randomly divided into two groups, namely the control group and the PFOS exposure group, with two males and two males in each group. Rats in the control group and the PFOS-exposed group should not be housed in one cage.

[0048] (2) Rats were fed for one week before the experiment. ...

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Abstract

The invention discloses a method for screening PFOS toxicity key genes and key signaling pathways by using transcriptomics. The method provided by the present invention first sets the blank control group and the PFOS exposure group of experimental animals and processes them to obtain biological samples, extracts the RNA of the obtained biological samples, and obtains the transcriptomic data of the biological samples through a high-throughput sequencer, and will obtain Bioinformatics analysis was performed on the sequencing data, including differential gene GO enrichment analysis, KEGG pathway enrichment analysis, protein interaction network analysis and GSEA analysis, and finally screened to obtain the results of PFOS toxicity hub genes and key signaling pathways. Applying the method provided by the present invention can help to identify the serious impact of PFOS on animals or humans and / or the toxicity mechanism of PFOS to animals or humans and to develop related drugs against PFOS toxicity.

Description

technical field [0001] The invention relates to the field of bioinformatics, in particular to a method for screening pivotal genes and key signaling pathways of perfluorooctane sulfonic acid toxicity by using transcriptomics. Background technique [0002] Perfluorooctane Sulfonate (PFOS) has been widely used in civil and industrial products, such as surfactants, fireproof coatings and photoresists due to its extremely low surface tension and unique physical properties of hydrophobicity and oleophobicity Wait. However, in view of the characteristics of such compounds as toxicity, environmental persistence, bioaccumulation and long-distance migration, the United Nations Environment Program listed PFOS and its salts and precursor compounds as new persistent substances in the "Stockholm Convention" in 2009. Organic Pollutants. Studies have shown that PFOS exhibits various toxic effects on animals, such as liver toxicity, testicular toxicity, pancreatic toxicity, neurotoxicity,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B5/00G16B30/00C12Q1/6869
CPCG16B5/00G16B30/00C12Q1/6869C12Q2535/122
Inventor 翁瑞王天润邱静
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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