Method for researching CXCR3-mediated TLRs/MyD88 signal path in promoting hepatitis B cirrhosis canceration

Abstract

The invention provides a method for researching a CXCR3-mediated TLRs / MyD88 signal path in promoting hepatitis B cirrhosis canceration. The method comprises the following steps of step III, extracting RNA and protein from LX-2 cells infected with a lentivirus, so as to carry out polymerase chain reaction (PCR) verification and western blot (WB) verification; and step IV, transfecting the LX-2 cells infected with the hepatitis B virus (HBV) lentivirus by using a CXCR3 interference agent, and extracting RNA and protein from the LX-2 cells transfected by the CXCR3 interference agent so as to carry out qPCR verification and WB verification. According to the method, by in-vitro culture of a hepatic stellate cell strain HBV-LX-2 for over-expressing HBV, and interfering expression of CXCR3 instantaneously, whether the CXCR3 participates in regulation and control of gene expression on the TLRs / MyD88 signal path or not and whether the CXCR3 has influence on proliferation, migration and apoptosis of the hepatic stellate cell or not are researched, thereby deducing the effect of the CXCR3 and the TLRs / MyD88 signal path in cirrhosis canceration. Research finds that the interference of the CXCR3 can down-regulate the gene expression on the TLRs / MyD88 signal path, so that the physiological activity of the HBV-LX-2 is inhibited.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a method for promoting the carcinogenesis of hepatitis B liver cirrhosis through CXCR3-mediated TLRs / MyD88 signaling pathway. Background technique [0002] According to the statistics of the World Health Organization, in 2015, an estimated 257 million people worldwide suffered from chronic hepatitis B, and chronic hepatitis B virus (HBV, HepatitisBvirus) infection can lead to liver cirrhosis and liver cancer [1,2,3]. 1.34 million people died from liver disease, including 740,000 people from liver cirrhosis [3] . HBV mainly causes chronic liver disease, which can last decades of chronic infection in patients, leading to liver inflammatory lesions. HBV does not directly cause liver cell lesions, but causes hepatitis and liver cancer through the host's immune and inflammatory responses. Chemokines and their receptors play an important role in the development of liver...

AI Technical Summary

Problems solved by technology

Chemokine receptor CXCR3 and TLRs / MyD88 signaling pathway play an important role in inducing hepatitis B to develop into cancer. In previous studies, the main researches were on the roles of CXCR3 or TLRs in cancer, and the relationship between the two There are few studies on the regulatory relationship between the development of liver cancer

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