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A palm-shaped rose-eared, fruity-flavored mushroom and its cultivation method

A cultivation method and technology of fruit-flavored mushrooms, applied in the field of microorganisms, can solve problems such as low yield and inability to commercialize production, and achieve the effects of fast nutrient conversion, shortening the fruiting cycle, and increasing yield

Active Publication Date: 2022-02-01
河南果菇蓉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this thesis, the mushroom is only artificially cultivated experimentally in the laboratory, and the output is very low, so it cannot be commercially produced.

Method used

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  • A palm-shaped rose-eared, fruity-flavored mushroom and its cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Separation and Identification of palm

[0034] In August 2020, the inventor Gur was collected to the palm Russia strain in Suhe City, Jilin Province. After tissue separation culture, observation of biological characteristics, test identification, identified as palm Silk body, preserved in the Normal Microbiology Center (CGMCC) of the China Microbial Square Safety Management Committee, the definition symbol is GGR-001, the address is No. 1, Beichen West Road, Chaoyang District, Beijing, China is CGMCN NO. 21941, Water Date It is April 2, 2021.

Embodiment 2

[0035] Example 2 Cultivation of the tube mother

[0036]Peel and wash 200g of potato pieces into the book, adding boiling water 1000mL 20min, filtered with gauze, whichever filtrate water and made up to 1000mL, Jia Ruqiong powder 22g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.8g, peptone 2.5 g, vitamin B1 and glucose 25g flour 0.5g, again heated until melted agar fully loaded tubes, each tube was charged in an amount of 1 / 5 of the length of the tube, installed plug sterile cotton plugs or plug of silica gel, plastic tied paper seal, test tubes every 7-10 bar bundle, tied up with a string; then placed in a high pressure steam autoclave, heated to 100 deg.] C, cold air is discharged, heated again to 0.1-0.12MPa under pressure (pot when the temperature reached 121 deg.] C) held sterilized 30min, when the pressure is reduced to be 0, the pot and slowly release the gas, while when not solidified medium, the tubes were placed into a ramp cooling to 20-24 deg.] C, to elimi...

Embodiment 3

[0037] Example 3 Solid expansion strain culture strains

[0038] Includes the following steps:

[0039] (1) The seed culture: solid medium was charged 400g of sorghum 500mL glass container, covered with a sterile gas permeable membrane, at 118-122 deg.] C, a pressure in the sterilization environment 0.12-0.14MPa 90-120min, inside the bottle to be cooled Between 22-24 deg.] C, under sterile conditions the access tube embodiment of mother Example 2, inoculation of 10cm 3 The tubes obtained in Example 3 of Mother, cultured under conditions 20-22 ℃ 40 days to obtain the original species;

[0040] The method of preparing the solid medium is Sorghum: in water at 100 ℃ added lime adjusted to pH 8.5, placed sorghum, sorghum top surface submerged 10cm, 20min to cook no white sorghum central inner core subject, remove sorghum, sorghum surface when dry air dry to a moisture content of 55% -60%, 1% of the dry weight of sorghum was added precipitated calcium carbonate, stirring evenly into a c...

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a palm-shaped fungus, a fruit-flavored mushroom and a cultivation method thereof. The palm-shaped fungus is preserved in the General Microbiology Center of the China Committee for the Preservation of Microorganisms, and the preservation number is CGMCC No.21941 . The method for cultivating fruit-flavored mushrooms includes the following steps: 1) Cultivation of the mother species in test tubes: sterilize the test tubes containing the culture medium of the mother species, insert the palm-shaped rosette tissue pieces under aseptic conditions, and store them at 20-22°C Cultivate under the conditions for 20‑25 days to obtain the test tube mother species; 2) Expanded culture strain: expand the test tube master species obtained in step 1) to obtain the expanded culture strain; 3) Fruiting culture: bag planting or Bottle planting step 2) Expand the cultivation of strains, produce mushrooms, and obtain finished products. The fruit-flavored mushroom obtained by the invention has high nutrient content, short cultivation time, and a biological conversion rate of over 70%, and can be widely used in industrialized production.

Description

Technical field [0001] The present invention belongs to the field of microbial technology, and specific relates to a palmatic rose, fruity mushroom and its cultivation method. Background technique [0002] The palm of the palm (net glass mushroom), the edge of the mushroom body to the inner curled umbromous cap and the lanyelle are the main characteristics, can be eaten, delicious, the bacteria is crisp, and the bacteria cover is rich High polysaccharide content, the bacteria part will naturally secrete gold-yellow sticky water beads at the beginning of growth, gradually tangerine and dark red, and the bacteria cover will exude a special fruit flavor when growing, and the normal people are normal. Snoving, 焯水 入 味 汤 汤 汤 汤 汤 汤 汤 汤 汤 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝 喝However, there are fewer types of palm, and there is no industrial manual planting, and there is no sales in the market, and the market is blank. [0003] Gravil Gur and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/40A01G18/20
Inventor 图力古尔李中魁
Owner 河南果菇蓉生物科技有限公司
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