Neural stem cell exosome nasal spray and preparation method thereof
A technology of neural stem cells and exosomes, applied in nervous system diseases, pharmaceutical formulations, aerosol delivery, etc., can solve problems such as high risk and overall cumbersomeness, and achieve good stability, improve dispersion, and reduce aggregation performance. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0037] Preparation: The arrangement of the neural stem cells exosomes is adopted as follows.
[0038] (1) Select 4 weeks of age SD rats, extract the femur using bone marrow cavity, and separate the bone marrow mesenchymal stem cells, and cultivate in the culture flask, replace it once every 2 days; wash away unsteadless wall cell. When the adhesive surface area is more than 80% in the culture flask, the ultra-low adhesion culture bottle is transferred and cultured for three days.
[0039] (2) Separation of suspension cells is separated, and the serum DMEM medium continues to cultivate for two days;
[0040] (3) Collecting the medium supernatant in the above-mentioned coating culture system, and after filtration with 0.22 μm filtrate, centrifuge at 4 ° C for 10 min, retain the supernatant;
[0041] (4) The supernatant obtained in step (3), centrifuged at 2000 × g for 100 min, retaining the supernatant;
[0042] (5) The supernatant obtained in step (4), centrifugation at 10,000 × g ...
Example Embodiment
[0047] Example 1, a neural stem cell exosomal nasal spray is configured as follows:
[0048] S1, according to the method of preparation, the preparation of the outer body;
[0049] S2, in the sub-suspension, according to the calculation amount, add a phosphate buffer solution having a concentration of 10 mm having a pH of 7.4 to the outer body, configured to 10 9The concentration of / ml and is blown to uniform and stored at 4 ° C. During the above process, the concentration of the outer body can be calculated by the Nanosight LM10 particle tracking analysis.
[0050] In step S2, a osmotic pressure regulator comprising 6% of the phosphoric acid buffered mass fraction in advance is added in advance, and mannitol is selected in the present application.
Example
[0051] Examples 2 to 27 and Comparative Examples 1 to 3, a neural stem cell epithelial nasal spray, and the difference from Example 1 is to adjust the type, amount of the pressure regulator, and the pH value of the buffer solution. Specifically, as shown in Table 1.
[0052] Table 1, Examples 1 to 27 and Comparative Examples 1 to 3 Basic Formulation Table
[0053]
[0054]
[0055] For Examples 1 to 27 and Comparative Examples 1 to 3, the stability was measured first, as shown in Table 2.
[0056] Table 2, Examples 1 to 27 and Comparative Example 1 to 3 Stability Experimental Data
[0057]
[0058]
[0059] In the above technical solution, in general, it can be considered that discoloration and precipitation means that a deterioration in which the outer body has deteriorated, which demonstrates sorbitol, mannitol, and sucrose as an osmotic regulator, which has better protection of exoc. The effect of the body has a good stability at 10 days and 4 ° C. Most samples show dif...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap