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Molecular identification marker primer combination for quantitative traits of apostichopus japonicus thorns and application method of molecular identification marker primer combination

A technology for molecular identification and quantitative traits, applied in the field of molecular biology

Inactive Publication Date: 2021-07-23
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thorn traits are determined by genetic factors and are also affected by various environmental factors (for example, temperature is one of the factors, and the number of spines in the same family of sea cucumbers varies in different temperature environments), and only the spine phenotype is used in the breeding process. For parental selection, spiny parents will be missed or wrongly selected

Method used

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  • Molecular identification marker primer combination for quantitative traits of apostichopus japonicus thorns and application method of molecular identification marker primer combination
  • Molecular identification marker primer combination for quantitative traits of apostichopus japonicus thorns and application method of molecular identification marker primer combination
  • Molecular identification marker primer combination for quantitative traits of apostichopus japonicus thorns and application method of molecular identification marker primer combination

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Embodiment 1

[0013] Example 1: Screening of markers

[0014] Taking the whole genome sequence of sea cucumber as a reference, the research carried out genome resequencing of 215 sea cucumbers from 8 representative regions, and obtained the SNP marker sites related to the number of warty feet of sea cucumber by comparison with the reference genome; Based on this, KASP primers were designed and genotyped, and verified in A. japonicus populations with differences in the number of verrucous feet, and finally a SNP primer sequence combination that can be used to identify the number of verrucous feet of A. japonicus was obtained. The specific method is as follows:

[0015] 1. Obtained by mark

[0016] 1.1 DNA sample extraction

[0017] Select 215 that are representative and in good condition, including Liaoning, Shandong, Russia (Vladivostok), the main producing areas of sea cucumbers in Liaoning, and the new species of sea cucumber "Shuiyuan No. 1". Among them, Lushun (the junction of the Ye...

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Abstract

The invention relates to a molecular identification marker primer combination for quantitative traits of apostichopus japonicus thorns and an application method of the molecular identification marker primer combination, and belongs to the field of molecular biology. The primer combination is shown as SEQ IP NO: 1-15, and the method specifically comprises the following steps: extracting DNA of an apostichopus japonicus sample by adopting an SDS method, carrying out SNP genetic typing on the sample to be detected by using a KASP combined marker, and detecting specific marker sites of the apostichopus japonicus with multiple thorns or few thorns; and the molecular identification standard of the spina polycalthroides is as follows: at least more than three spina markers are covered with each individual. The method can determine the number of the apostichopus japonicus thorns from the genetic perspective.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a combination of marker primers for quantitative identification of sea cucumber (A. japonicus) spines and an application method thereof. Background technique [0002] Molecular markers refer to heritable and detectable DNA sequences or proteins, and molecular markers refer to specific DNA fragments that can reflect certain differences in the genomes of individual organisms or populations. Compared with morphological markers, biochemical markers, and cytological markers used in traditional breeding, DNA molecular markers have significant advantages, and they are not affected by individual development and environment. Molecular marker-assisted breeding utilizes the characteristic that molecular markers are closely linked with genes that determine target traits. By detecting molecular markers, it is possible to know whether the target gene exists and achieve the purpose ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 丁君常亚青高萍萍王荦
Owner DALIAN OCEAN UNIV