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Nucleic acid composition for detecting canine coronavirus and canine parvovirus, kit and application

A technology of nucleic acid composition and canine parvovirus, which is applied in the field of pathogen detection, can solve the problems of low detection sensitivity and single pathogen of colloidal gold method, and achieve the effect of good specificity and high sensitivity

Pending Publication Date: 2021-07-23
上海基灵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the patent CN201711054378.X provides a rapid quantitative detection card for canine coronavirus antibody and its application method. The colloidal gold method has the problem of low detection sensitivity; although the sensitivity of ordinary PCR is high, the pathogen detected is single

Method used

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  • Nucleic acid composition for detecting canine coronavirus and canine parvovirus, kit and application
  • Nucleic acid composition for detecting canine coronavirus and canine parvovirus, kit and application
  • Nucleic acid composition for detecting canine coronavirus and canine parvovirus, kit and application

Examples

Experimental program
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Effect test

Embodiment 1

[0050] This embodiment provides a nucleic acid composition for detecting canine coronavirus (CCV) and canine parvovirus (CPV), which includes the first nucleic acid composition for detecting canine coronavirus and the second nucleic acid composition for detecting canine parvovirus nucleic acid composition. Wherein, the first nucleic acid composition includes primer pair 1 and probe 1, and the second nucleic acid composition includes primer pair 2 and probe 2. The nucleotide sequence of the primer pair 1 is shown in SEQ ID NO.1-2, the nucleotide sequence of the probe 1 is shown in SEQ ID NO.3, and the nucleotide sequence of the primer pair 2 is shown in SEQ ID NO. 4-5, the nucleotide sequence of probe 2 is shown in SEQ ID NO.6.

[0051] The sequence is as follows:

[0052]

[0053] The above-mentioned nucleic acid combination is obtained by searching relevant documents of the two pathogens and analyzing and screening related genes. The whole genome sequence of canine coro...

Embodiment 2

[0055] This embodiment provides a detection kit, which includes the nucleic acid composition provided in Embodiment 1. The kit provided in this example uses a 25 μL reaction system, including 12.5 μL of PCR reaction solution (including reverse transcriptase, Taq DNA polymerase, dNTP, MgCl 2 );

[0056] Canine coronavirus primer probe set: 0.15 μL of CCV-F, 0.2 μL of CCV-R, 0.2 μL of CCV-P;

[0057] Primer-probe set for canine parvovirus: 0.15 μL of CPV-F, 0.15 μL of CPV-R, 0.2 μL of CPV-P, 3.7 μL of DEPC-treated water, and 5 μL of template. The PCR reaction solution was purchased from Zhuhai Baorui Biotech.

Embodiment 3

[0059] This embodiment provides a detection reagent, which includes the nucleic acid composition provided in Embodiment 1.

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Abstract

The invention discloses a nucleic acid composition for detecting canine coronavirus and canine parvovirus, a kit and application, and relates to the technical field of pathogen detection. The composition comprises a first nucleic acid composition for detecting canine coronavirus and a second nucleic acid composition for detecting canine parvovirus, the composition can be used for real-time fluorescent quantitative PCR detection of canine digestive tract virus, and the inventor verifies that the nucleic acid composition is good in pathogen detection specificity and high in sensitivity. The lowest detection limits of the two pathogens reach 5 * 10 < 2 > copies / mL, and early diagnosis or treatment of affected dogs is facilitated. Corresponding detection kits and detection reagents can be prepared based on the nucleic acid composition.

Description

technical field [0001] The invention relates to the technical field of pathogen detection, in particular to a nucleic acid composition, kit and application for detecting canine coronavirus and canine parvovirus. Background technique [0002] Viral enteritis is one of the most common causes of infectious diarrhea in dogs under 6 months of age. Canine coronavirus (CCoV or CCV) and canine parvovirus (Canine Parvovirus, CPV) have been identified as the main pathogens causing canine viral enteritis. Among them, canine parvovirus is highly contagious, has a high incidence rate, and has strong resistance to the environment and common disinfectants. The canine coronavirus is a single-stranded RNA virus with an envelope, which is more sensitive to heat and detergents. After the dog is infected with CCV or CPV, it can show different degrees of gastrointestinal symptoms. Vomiting is the main symptom at the initial stage. As the disease progresses, mushy, semi-pasty, watery diarrhea c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11
CPCC12Q1/701C12Q1/6851C12Q2531/113C12Q2563/107Y02A50/30
Inventor 孙依欣张晓婷
Owner 上海基灵生物科技有限公司
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