Photoprotective compositions containing malassezia-derived compounds and/or chemical analogs thereof

A technology of compounds and analogs, applied in the field of photoprotective compositions containing compounds derived from Malassezia (MALASSEZIA) and/or their chemical analogs, capable of solving problems such as harmful ingredients

Pending Publication Date: 2021-07-23
VERSICOLOR TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many commercially available skin lightening products, while effective to varying degrees, contain harmful ingredients, some of which have been linked to cancer

Method used

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  • Photoprotective compositions containing malassezia-derived compounds and/or chemical analogs thereof
  • Photoprotective compositions containing malassezia-derived compounds and/or chemical analogs thereof
  • Photoprotective compositions containing malassezia-derived compounds and/or chemical analogs thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0256] Compound name

[0257] Table 1 below shows the structures and names of the compounds of the present invention.

[0258] Table 1

[0259]

[0260]

[0261]

[0262]

[0263]

[0264]

[0265]

[0266]

Embodiment 2

[0268] Apoptosis Inducing Activity of Indirubin and Indirubin Derivatives

[0269] Reagent

[0270] Alexa Fluor 488 Annexin V / Dead Cell Apoptosis Kit, Fetal Bovine Serum (FBS), 0.25% Trypsin-EDTA (1×), Caspase-Glo 3 / 7 Assay, RPMI 1640 Medium , Dulbecco's Modified Eagle Medium and antibiotic antimycotic solution (100×).

[0271] The cell line MeWo( HTB-65 TM ), WM115 ( CRL-1675) and B16F1 ( CRL-6323) were maintained in the following media: media for MeWo and B16F1: DMEM supplemented with 10% FBS; media for WM115: RPMI 1640 supplemented with 10% FBS.

[0272] experimental method

[0273] Cells were harvested and cell numbers determined using a Countess cell counter. Dilute the cells to the desired density with culture medium. For example, the final cell density can be 4,000 cells / well for 6 hour and 24 hour treatments, and 2,000 cells / well for 48 hour and 72 hour treatments. For Annexin V assays, 384-well clear-bottom plates (Corning 3712) were used, while for Casp...

Embodiment 3

[0280] Cell Viability After Exposure to Indirubin and Indirubin Derivatives

[0281] Reagent

[0282] CellTiter- 2.0 Analysis.

[0283] experimental method

[0284] For CellTiter-Glo assays, test compounds were prepared in 10 mM DMSO solutions. Compounds were serially diluted into 12 concentrations. 40 μL of cells from the 100,000 cells / ml suspension was dispensed into each well of a 384-well plate (Corning3570). Incubate the plate at 37 °C, 5% CO 2 and 95% humidity overnight. Test compounds were added, with DMSO as vehicle control. Incubate the plate at 37 °C, 5% CO 2 and 95% humidity for 6, 24 or 48 hours, and 40 μL of CellTiter-Glo reagent was added to the wells to analyze cell viability.

[0285] result

[0286] Compounds and compositions of the invention, including indirubin and its chemical analogs, are expected to induce cell death. Chemical analogues of indirubin are expected to exhibit, for example, stronger apoptosis-inducing activity than indirubin. Lik...

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PUM

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Abstract

The present invention relates to compounds, compositions, and methods for modulating skin pigmentation and treating or preventing UV-induced skin damage, erythema, aging of the skin, sunburn, and hyperpigmentation in a subject. The compounds, compositions, and methods of the present invention generally involve Malassezia- derived compounds, including malassezin and indirubin, and / or chemical analogs thereof. Other applications of the compounds and compositions disclosed herein include, but are not limited to, improving hyperpigmentation caused by a hyperpigmentation disorder, inducing melanocyte apoptosis, and modulating arylhydrocarbon receptor (AhR) activity, melanogenesis, melanin production, melanosome biogenesis, melanosome transfer, melanocyte activity, and melanin concentration.

Description

[0001] Cross References to Related Applications [0002] The present invention claims U.S. Provisional Application No. 62 / 685,800, filed June 15, 2018, U.S. Provisional Application No. 62 / 686,912, filed June 19, 2018, U.S. Provisional Application No. 62, filed August 24, 2018 / 722,412 and U.S. Provisional Application No. 62 / 742,657, filed October 8, 2018. The entire contents of the aforementioned applications are incorporated by reference. In addition, U.S. Provisional Application No. 62 / 306,468 filed March 10, 2016, U.S. Provisional Application No. 62 / 656,769 filed April 12, 2018, U.S. Patent Application No. 62 / 668,007, U.S. Patent Application No. 15 / 455,932 filed March 10, 2017, now U.S. Patent No. 10,131,631, U.S. Patent Application No. 16 / 382,891 filed April 12, 2019 and May 7, 2019 The entire contents of US Patent Application Serial No. 16 / 405,127, filed on , are hereby incorporated by reference herein. [0003] field of invention [0004] The present invention relate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04A61K8/49A61Q19/02C07D209/12A61P17/00A61K31/404
CPCC07D209/12C07D487/04A61P17/00A61Q19/02A61K8/492A61K31/40A61K9/0014A61K9/12A61K8/4913A61P17/16A61Q19/08A61Q17/04A61K31/404A61P19/02C07D403/04C07D209/34
Inventor M·因泽格A·M·辛普森
Owner VERSICOLOR TECH LLC
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