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Method for promoting germination of cymbidium goeringii seeds

A technology of seeds and Chunlan, which is applied in the field of botany, can solve the problems of rising temperature of the seed embryo, poor practicability, difficulties, etc., and achieve the effects of promoting seed germination, improving impermeability, and improving impermeability

Active Publication Date: 2021-07-27
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the physical method, it is very difficult to cut the seed coat with ophthalmic scissors or surgical blades because the seeds are very small, and the operability is not strong, so it cannot be widely used; the seed coat is broken by ultrasonic vibration, not only the seed coat is not easy to break, but it is easy to make the seed coat on the contrary. The temperature of the embryo part rises, the embryo tissue is damaged, and the seeds are browned
In the chemical method, using NaOH solution and NaCIO solution to corrode the seed coat has a certain effect, but NaOH solution and NaCIO solution have certain damage to the seed embryo. In order to remove NaOH solution and NaCIO solution, it must be washed several times with sterile water. Filtration, difficult to operate, not very practical

Method used

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  • Method for promoting germination of cymbidium goeringii seeds
  • Method for promoting germination of cymbidium goeringii seeds
  • Method for promoting germination of cymbidium goeringii seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Cultivate capsules:

[0047]The Chunlan variety "Cuigaihe" was used as the female parent, and the Chunlan variety "Global Heding" was used as the male parent to carry out artificial hybridization and cultivate capsules. After the Chunlan variety "Cuigaihe" bloomed for 5 days, the nose and pollen pieces were removed, and the style was covered with a small white gauze bag to avoid insect pollination. "The pollen block of "Global Lotus Ding" is fed into the stamen cavity under the nose. After pollination, the style is continued to be covered with a small white gauze bag to prevent repeated pollination by insects and ensure the success rate of hybridization. The small gauze bag is removed after the seeds are fertilized and expanded, and cultivated normally, the capsules continue to expand, and the capsules are obtained.

[0048] 2. Disinfection treatment:

[0049] Step 1, fold off the capsule, peel off the remaining petals, wipe the surface of the capsule with 75% alco...

Embodiment 2

[0052] S1 configures nutrient solution and culture solution, wherein the nutrient solution includes 0.3mg / L 1 / 2MS+NAA medium, 20g / L sucrose and 0.5g / L peptone, the pH of the nutrient solution is 5.4; the culture solution includes 0.3mg / L 1 / 2MS+NAA medium, 20g / L sucrose, 0.5g / L activated carbon and 0.5g / L peptone, the pH of the culture solution is 5.4;

[0053] S2 Take 0.1 g of the seeds obtained in Example 1 and put them into a 150 mL sterile syringe, use the syringe to inhale 100 mL of nutrient solution and 0.02 mL of Tween 80, shake horizontally for 3 minutes, and stand vertically for 12 hours after the shaking is completed to obtain treatment solution A;

[0054] S3 Drain the upper liquid of the treatment solution A from the mouth of the syringe, and then put a sterile needle filter on the mouth of the syringe to press and filter out other treatment solutions to obtain seeds, add 100mL of culture solution into the syringe, shake horizontally for 1min, and obtain the treatmen...

Embodiment 3

[0058] S1 configures nutrient solution and culture solution, wherein the nutrient solution includes 0.2mg / L 1 / 2MS+NAA medium, 25g / L sucrose and 0.6g / L peptone, the pH of the nutrient solution is 5.1; the culture solution includes 0.2mg / L 1 / 2MS+NAA medium, 25g / L sucrose, 0.4g / L activated carbon and 0.6g / L peptone, the pH of the culture solution is 5.1;

[0059] S2 Take 0.3g of the seeds obtained in Example 1 and put them into a 150mL sterile syringe, use the syringe to inhale 100mL of nutrient solution and 0.03mL of Tween 80, shake horizontally for 3min, and stand vertically for 12h after the shaking is completed to obtain treatment solution A;

[0060] S3 Drain the upper liquid of the treatment solution A from the mouth of the syringe, and then put a sterile needle filter on the mouth of the syringe to press and filter out other treatment solutions to obtain seeds, add 100mL of culture solution into the syringe, shake horizontally for 1min, and obtain the treatment solution B ...

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Abstract

The invention discloses a method for promoting germination of cymbidium goeringii seeds. The method comprises the following steps of disinfecting cymbidium goeringii capsules; placing seeds in a sealed sterilization container, adding a nutrient solution and Tween 80, performing horizontal oscillation, and vertically standing after oscillation to obtain a treatment solution A; taking out the seeds from the treatment solution A, placing the seeds in the sealed sterilization container, adding a culture solution, and performing horizontal oscillation to obtain a treatment solution B; performing negative pressure pumping treatment on the treatment solution B, and discharging bubbles of seed coats to obtain a treatment solution C; and pouring the treatment solution C into a culture bottle for seed culture. The Tween 80 and the nutrient solution are matched to treat seed coats, the Tween 80 can effectively improve the non-hydrophilicity of the seed coats, contact between the nutrient solution and the seed coats is improved, and the watertightness of the seed coats is improved; and then the seeds are placed in the culture solution and subjected to negative pressure treatment, the bubbles of the seed coats are discharged, the culture solution synchronously enters the seed coats and makes full contact with embryonic cells, and seed germination is effectively promoted.

Description

technical field [0001] The invention relates to the technical field of botany, in particular to a method for promoting the germination of Chunlan seeds. Background technique [0002] Chunlan is a perennial herb of Orchidaceae, Orchid genus, and is a representative species of Chinese orchids. It is native to the Yangtze River Basin or Southwest China, and is also distributed in Japan and the Korean Peninsula. Chunlan has beautiful leaves, elegant plant shape, quiet fragrance and rich petals. The natural flowering period is around the Spring Festival. Compared with other orchids that are not resistant to cold, Chunlan has obvious advantages as a high-end potted flower and New Year's Festival flower. The development potential of Chunlan industry is huge, but my country's Chunlan has traditionally formed the habit of emphasizing varieties and ignoring large-scale breeding and production. Its value orientation is also based on rarity. There are almost no varieties suitable for or...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 孙叶刘春贵魏小羽李风童马辉单东方弓剑陶凡
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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