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Antigen peptide related to colorectal cancer driver gene mutation and application thereof

A colorectal cancer and driver gene technology, applied in the field of biomedicine, can solve the problems of superior therapeutic agents, which have not been systematically discovered and established, and have failed to form effects, etc., to achieve inhibition of tumor cell growth, good clinical transformation and The effect of disease prevention prospects

Inactive Publication Date: 2021-07-27
深圳市新靶向生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, because technology requires multidisciplinary, high-tech, and multi-platform collaborative research, antigenic peptides related to colorectal cancer driver gene mutations have not been systematically discovered and a relatively complete peptide library has not been established, so the effect has not yet been formed. Superior Healing Agent

Method used

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  • Antigen peptide related to colorectal cancer driver gene mutation and application thereof
  • Antigen peptide related to colorectal cancer driver gene mutation and application thereof
  • Antigen peptide related to colorectal cancer driver gene mutation and application thereof

Examples

Experimental program
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Embodiment Construction

[0017] Below, in conjunction with specific embodiment, the present invention is described further:

[0018] Antigen peptides related to colorectal cancer driver gene mutations used in the examples are shown in Table 1:

[0019] Table 1 Antigenic peptide sequences associated with colorectal cancer driver gene mutations

[0020]

[0021] The operation of establishing antigen peptide-specific CTL clones related to colorectal cancer driver gene mutations is as follows:

[0022] 10 from the same healthy donor 5 CD8 + T cells loaded with 10 antigenic peptides associated with colorectal cancer driver mutations 4 Mo-DCs were stimulated twice at intervals of 1 week, and then passed autologous 10 5 Mitomycin C-treated PBMCs loaded with antigenic peptides associated with colorectal cancer driver gene mutations were stimulated once and screened by standard cytotoxicity assays.

[0023] T2 cells were loaded with 5uM antigenic peptides associated with colorectal cancer driver mutati...

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PUM

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Abstract

The invention discloses an antigen peptide related to colorectal cancer driver gene mutation. The antigen peptide related to colorectal cancer driver gene mutation is related to mutation of at least two genes in PIK3CA, APC, PCBP1, NRAS, SMAD2, FBXW7, CTNNB1 and KMT2D. The sequences of the antigen peptides related to colorectal cancer driver gene mutation are at least two of SEQ No.1-16; according to the application of the antigen peptide, the antigen peptide is used for inducing generation of specific cytotoxic T cell clone; and the antigen peptide has high affinity with MHC I molecules on DC cells, can effectively stimulate and induce generation of specific cytotoxic T lymphocytes, can be used for immune clearance of tumor cells with driving gene mutation related to colorectal cancer, and has good treatment potential.

Description

technical field [0001] The invention relates to an antigenic peptide related to colorectal cancer driver gene mutation and application thereof, belonging to the technical field of biomedicine. Background technique [0002] Colorectal cancer is one of the top five cancers with a high genetic tendency. 50% of the offspring whose parents have colorectal cancer insist on colonoscopy every year. Colorectal cancer (CRC) is the second leading cause of cancer-related death in Western countries. CRC arises from the colorectal epithelium due to genetic alterations in defining oncogenes and tumor suppressor genes (TSGs). In studies of CRC, two major mechanisms of genome instability have been identified. The first, called chromosomal instability (CIN), results from a series of genetic changes involving the activation of oncogenes such as K-ras and inactivation of TSGs such as p53, DCC / Smad4 and APC. The second, termed microsatellite instability (MSI), is caused by inactivation of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N9/12C12N5/0783A61K39/00A61P35/00
CPCA61P35/00A61K39/001102A61K39/001162C07K14/4748C12N5/0638C12N9/1205
Inventor 郝冰娜李许峰蔡睿赵环罗夫辛克纳吉张积仁
Owner 深圳市新靶向生物科技有限公司
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