Inactivated virus preserving fluid formula and preparation method thereof

Abstract

The invention discloses an inactivated virus preserving fluid formula and a preparation method thereof, and the inactivated virus preserving fluid formula comprises the following components by weight: 5-20 g of a protein denaturant: one or a combination of more of high-concentration guanidine salt, guanidine hydrochloride and guanidine isothiocyanate; 0.5-2g of RNA (Ribonucleic Acid) enzyme inhibitor; a buffer stabilizer: 2-6 g of Tris; a surfactant: 1-4 g of sodium dodecyl sarcosinate; 0.001-0.01 g of an acid-base indicator; 0.2-1 g of an anti-freezing agent; the RNA enzyme inhibitor is one or a combination of more of EDTA (Ethylene Diamine Tetraacetic Acid) and guanidine isothiocyanate; the acid-base indicator is one of basic fuchsin and neutral fuchsin; the preserving fluid is safe and non-toxic, can keep a liquid state at a low temperature, is convenient to sample, can inactivate viruses and protect RNA enzyme degraded viruses, can resist growth of bacteria and fungi, is not easy to cause pollution, can be preserved for a long time and detect viruses, enables the viruses not to be infectious, and avoids the risk that transportation and detection personnel are infected.

Description

technical field [0001] The invention relates to the technical field of making an inactivated virus preservation solution, in particular to a formula of an inactivated virus preservation solution and a preparation method thereof. Background technique [0002] The 2019 novel coronavirus (2019-nCoV) is a new strain of coronavirus discovered in humans in 2019. It belongs to the genus Betacoronavirus. Common signs after people are infected with the virus include respiratory symptoms, fever, cough, shortness of breath and dyspnea. etc., and in more severe cases, infection can lead to pneumoniae respiratory syndrome, kidney failure, and even death. The latest research results reveal that the transmission characteristics of 2019-nCoV are highly contagious and highly concealed. Whether carrying 2019-nCoV has become one of the key clinical indicators for judging whether it is infected. Clinically, two 2019-nCoV nucleic acid tests are negative as a cure standard. [0003] In the "Tec...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the virus preservation solution commonly used in the collection of new coronavirus samples is an isotonic saline solution or phosphate buffer solution that can preserve virus activity. Although cells can be preserved to protect virus activity, it cannot resist the growth of bacteria and fungi. It is easy to cause pollution and affect long-term storage and virus detection. At the same time, active viruses are infectious, which puts transportation and testing personnel at risk of infection, especially the highly contagious and highly pathogenic 2019-nCoV; on the other hand, 2019-nCoV infection reaches its peak in autumn and winter, and the existing virus preservation solution is easily frozen into a solid state in this environment, which affects the sampling problem of clinical medical personnel; the formula of the inactivated virus preservation solution and its production method of the present invention, The preservation solution is safe and non-toxic, it can maintain a liquid state at low temperature, it is convenient for sampling, it can inactivate viruses and protect RNase from degrading viruses