Method for detecting isoquinoline alkaloids in honey

Abstract

The invention discloses a method for detecting isoquinoline alkaloids in honey, which comprises the following steps of: taking a honey sample containing isoquinoline alkaloids, dissolving the honey sample in water of which the amount is 10-30 times that of the honey, adding hydrochloric acid, and stirring well to prepare a test solution; preparing the honey from which the isoquinoline alkaloids are removed into a blank solution according to the scheme; adding the blank solution into a transparent container, releasing a light source at one end of the transparent container, and detecting the illumination intensity L0 at the other end of the transparent container; adding a test solution into a transparent container, releasing a light source at one end of the transparent container, detecting the illumination intensity L1 at the other end of the transparent container, and applying ultrasonic waves to the test solution during detection; calculating the absorbance of the test solution according to a formula that the absorbance is equal to log (I0 / I1); and calculating the content of the isoquinoline alkaloid components in the honey by inquiring an absorbance-isoquinoline alkaloid standard curve.

Description

technical field [0001] The present invention relates to the field of honey. Background technique [0002] Honey is the nectar or secretions that bees take from the flowers of plants with a water content of about 75%, and store them in their second stomach. When the substances and amino acids are enriched to a certain value, at the same time, the polysaccharides in the nectar are transformed into simple sugars glucose and fructose that can be directly absorbed by the human body. Honey is a supersaturated solution of sugar, which will crystallize at low temperature. The crystallized part is glucose, and the non-crystallized part is mainly fructose. [0003] Honey contains a variety of active ingredients, such as fructose, glucose, etc., but in the process of honey formation, it is inevitable to form some toxic and harmful substances, such as antibiotics, pesticides, alkaloids, etc. Excessive amounts of these substances , Seriously affect the quality of honey and the safety o...

AI Technical Summary

Problems solved by technology

[0003] Honey contains a variety of active ingredients, such as fructose, glucose, etc., but in the process of honey formation, it is inevitable to form some toxic and harmful substances, such as antibiotics, pesticides, alkaloids, etc. Excessive amounts of these substances , seriously affecting the quality of honey and the safety of consumers

[0004] However, in the existing technology, the detection of these toxic and harmful substances is usually through the method of laboratory sampling, which is complicated and cumbersome.