Application of knocking-down ARID1A in inhibition of retinal ganglion cell apoptosis after injury

A technology of apoptosis and optic nerve injury, applied in the field of biomedicine, can solve problems such as hindering the survival of CNS neurons

Inactive Publication Date: 2021-08-06
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Numerous studies in the past few decades have mostly focused on the factors that hinder the survival of CNS neurons in the external environment. However, the use of gene therapy or drug therapy to reduce the factors that are not conducive to the survival of nerves in the environment can only allow limited retinal ganglion survival. cell survival

Method used

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  • Application of knocking-down ARID1A in inhibition of retinal ganglion cell apoptosis after injury
  • Application of knocking-down ARID1A in inhibition of retinal ganglion cell apoptosis after injury
  • Application of knocking-down ARID1A in inhibition of retinal ganglion cell apoptosis after injury

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Experimental program
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Effect test

Embodiment 1

[0040] Example 1. ARID1A gene is a gene intervention target to promote the survival of retinal ganglion cells after optic nerve injury

[0041] This example provides a gene intervention target that can promote the survival of retinal ganglion cells after optic nerve injury. Arid1a Fl / Fl Mice are used as models, and the ARID1A gene in RGCs is specifically knocked out using the cre-Loxp system and AAV-Cre virus eye-point injection technology. The steps are as follows:

[0042] 1. Preparation of virus

[0043] The virus used to knock out the ARID1A gene in RGCs was AAV-Cre virus, and AAV-GFP virus was used as a control. AAV-Cre virus and AAV-GFP virus are products of Shandong Weizhen Company, both of which are recorded in the literature (Kevin KyungsukPark et al., Promoting Axon Regeneration in the Adult CNS by Modulation of the PTEN / mTOR Pathway, SCIENCE VOL 322 7NOVEMBER 2008).

[0044] 2. Detection of the effect of knockout of ARID1A gene on RGC

[0045] (1) Determination ...

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Abstract

The invention discloses an application of knocking down ARID1A in inhibition of retinal ganglion cell apoptosis after injury. The invention finds that the ARID1A gene knockout can inhibit the RGC apoptosis of optic nerve injury animals, and the survival rate of the RGC after the ARID1A gene knockout is obviously increased compared with that of the RGC without the ARID1A gene knockout, so that the ARID1A gene knockout substance can be used as a protective agent of retinal ganglion cells to treat optic nerve injury. The invention provides a new gene intervention target spot to effectively inhibit the apoptosis of the retinal ganglion cells after optic nerve injury, and provides a strategy for repairing optic nerve injury, searching a treatment method for optic nerve injury to relieve the pain of a patient and restore the neurological function.

Description

technical field [0001] The invention relates to the application of knocking down ARID1A in inhibiting retinal ganglion cell apoptosis after injury in the field of biomedicine. Background technique [0002] In the field of modern medicine, treating nerve injuries, especially those of the central nervous system (CNS) is still a huge challenge. The traditional concept is that the adult mammalian central nervous system is difficult to regenerate after injury, such as optic nerve injury, the axon and dendrite growth and regeneration ability of the injured neurons are extremely poor, and it is difficult to form new synaptic connections. Optic nerve injury is a common type of eye trauma and the cause of blindness. It is more common in various facial, craniocerebral and orbital traumas. For example, compression, traction, tearing, cutting and other reasons can lead to severe damage to the optic nerve, resulting in visual impairment of patients. Irreversible loss of function. After...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K35/76A61P25/00A61P27/02
CPCA61K35/76A61K45/00A61P25/00A61P27/02
Inventor 刘长梅彭雪琦杨树广刘佩佩杜洪震滕兆乾
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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