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Spinoside, preparation thereof and application of spinoside in preparation of hypoglycemic drugs

A technology of jujube seed saponin and jujube seed, which is applied in the direction of drug combination, medical formula, steroidal compounds, etc., can solve the problems of less research on the activity of jujube seed saponin, and achieve the effects of low toxic and side effects, increased proliferation, and low dosage

Active Publication Date: 2021-08-06
汉方萃取生物科技(海南)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sour jujube saponin is one of the main chemical components in sour jujube seed. At present, the research on the pharmacological activity of sour jujube saponin at home and abroad is mainly sedative and hypnotic, while there are few studies on other activities of sour jujube saponin.

Method used

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  • Spinoside, preparation thereof and application of spinoside in preparation of hypoglycemic drugs
  • Spinoside, preparation thereof and application of spinoside in preparation of hypoglycemic drugs
  • Spinoside, preparation thereof and application of spinoside in preparation of hypoglycemic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]Example 1 Prepare Semen Semen Spinosae Alcohol Extract from Semen Semen Semen Semen Semen

[0050] (1) Put the jujube seed (Citrus aurantium L.var.amara Engl) in an electric blast drying oven at 45°C and dry for 24 hours, pulverize it with a pulverizer, pass it through a 40-mesh sieve, and obtain coarse powder of the jujube kernel for later use;

[0051] (2) Weigh 200g of Suanzaoren coarse powder into a container, add 3000mL of 75% ethanol aqueous solution, shake well, heat and reflux in a water bath at 80°C for extraction twice, heating for 3 hours each time; filter with suction, take the filtrate, and combine the filtrates , the filtrate was concentrated under reduced pressure with a rotary evaporator at 45° C., and dried in a vacuum oven at 45° C. to constant weight to obtain the ethanol extract of Jujube Semen.

Embodiment 2

[0052] Example 2 Preparation of Semen Semen Semen Semen Semen Semen Semen Semen Semen Semen Spinosae Alcohol Extract

[0053] 1) Put the jujube seed (Citrus aurantium L.var.amara Engl) in an electric blast drying oven at 50°C and dry for 18 hours, pulverize it with a pulverizer, pass it through a 18-mesh sieve, and obtain coarse powder of the jujube kernel for later use;

[0054] (2) Weigh 300g of Suanzaoren coarse powder into a conical flask, add 3000mL of 70% ethanol, fully oscillate and shake well, heat and reflux extraction twice in a water bath at 80°C, heating for 3 hours each time; use Buchner funnel to suction filter, Take the filtrate, combine the filtrates, concentrate the filtrate under reduced pressure at 45° C. with a rotary evaporator, and dry it in a vacuum oven at 45° C. to constant weight to obtain the ethanol extract of Jujube Semen.

Embodiment 3

[0055] Example 3 Prepared the first-class saponin SZR I from Semen jujube Semen

[0056] Take 30 g of the jujube seed ethanol extract in Example 1 and dissolve it into a suspension with 800 mL of distilled water, transfer the suspension to a separatory funnel, add an equal volume of petroleum ether, shake, and let stand for 30 minutes, until the two phases After complete separation, the upper petroleum ether layer was discarded, and the water layer was set aside for use, and repeated 3 times. Add an equal volume of ethyl acetate to the water layer, shake it, and let it stand for 30 minutes. After the two phases are completely separated, discard the upper layer extract, collect the water layer, and repeat 3 times. Add the same volume of water-saturated n-butanol to the water layer, shake it, and let it stand for 30 minutes. After the two phases are completely separated, rotate the piston of the separatory funnel to collect the extract of the upper layer, extract three times, an...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and discloses jujuboside, preparation thereof and application of the jujuboside in preparation of hypoglycemic drugs. The method comprises the following steps: 1) heating, refluxing and extracting spina date seeds by adopting an ethanol water solution to obtain a spina date seed ethanol extract; mixing the spina date seed ethanol extract with water, extracting with petroleum ether, ethyl acetate and n-butyl alcohol, collecting n-butyl alcohol phase extract liquor, concentrating and drying to obtain spina date seed primary saponin; or (2) purifying the spina date seed primary saponin, sequentially carrying out gradient elution by using water and ethanol solutions with different concentrations, and concentrating and drying eluent obtained by eluting by using a 50% ethanol solution to obtain spina date seed secondary saponin. The spina date seed saponin is spina date seed primary saponin and / or spina date seed secondary saponin. The jujuboside is applied to preparation of hypoglycemic drugs. The jujuboside can be used for remarkably inhibiting alpha-glucosidase and alpha-amylase and improving HepG2 cell insulin resistance so as to play a role in reducing blood sugar.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to jujube seed saponin, its preparation and its application in the preparation of hypoglycemic drugs. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia caused by multiple etiologies. Diabetes is now divided into four types, type I diabetes, type II diabetes, other special types of diabetes, and gestational diabetes; among them, type II diabetes is the most common. Type II diabetes can lead to various complications such as diabetic nephropathy, diabetic mental illness, and diabetic retinopathy. In addition, type II diabetes can also increase the level of lipoproteins, cause coronary heart disease and stroke and other vascular diseases, and seriously endanger human health. [0003] Elevated blood sugar after meals and insulin resistance are important factors leading to the onset of type II d...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/7048A61K36/725A61P3/10
CPCC07J71/0005A61K36/725A61P3/10A61K2236/17A61K2236/333A61K2236/35A61K2236/33A61K2236/55
Inventor 姜建国谢红钟瑞芳李艺萌
Owner 汉方萃取生物科技(海南)股份有限公司
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