Kit for extracting putrefying bacteria DNA (deoxyribonucleic acid) in conditioning bag and extraction method thereof

Abstract

The invention discloses a kit for extracting putrefying bacteria DNA (deoxyribonucleic acid) in a conditioning bag and an extraction method thereof. The kit comprises a sample cleaning solution A, a bacterial lysis solution B, a bacterial lysis solution C and a bacterial lysis solution D, wherein the sample cleaning solution A is mainly prepared from Na2HPO4, NaCl and NaH2PO4.2H2O; the bacterial lysis solution B is mainly prepared from Tris, Na2EDTA and lysozyme; the bacterial lysis solution C is mainly prepared from SDS and protease K; and the bacterial lysis solution D is mainly prepared from Tris-HCl, NaCl, EDTA and CTAB. According to the kit and the extraction method thereof, through the steps of physical cleaning of the sample cleaning solution A and filtering treatment of a first filter, the subsequent extraction efficiency can be greatly improved, the problem that a filtering column is blocked in the subsequent steps can be effectively solved, and compared with a traditional method that culture is conducted firstly and then identification is conducted, the identification time of the putrefying bacteria in the conditioning bags is greatly shortened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for extracting DNA of spoilage bacteria in a conditioning bag and an extraction method thereof. Background technique [0002] With the rapid development of convenience food, delicious convenience food has become more and more popular among consumers, and the core of its deliciousness is rich and varied, delicious and nutritious conditioning packs. High-pressure sterilized conditioning packs are popular because they do not contain preservatives, and because they do not contain preservatives, under normal temperature conditions, if the packaging is damaged, it will be easily contaminated by microorganisms, and the risk of spoilage and swelling will also be greatly increased. Therefore, whether the rapid detection of spoilage microorganisms in the conditioning package can be realized is the technical key to further cause analysis, and it is also crucial to the quality ...

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is that the DNA extraction kit and its extraction method in the prior art are easy to block the filter column with the nucleic acid adsorption membrane when extracting the spoilage bacteria DNA in the conditioning bag, the extraction rate of DNA is not high, and the DNA after extraction There are too many inclusions affecting PCR amplification, affecting subsequent research or diagnosis, and the problem is expensive and the extraction cycle is long

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