Hepatotoxic component of airpotato yam and determination method thereof
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A technique for determining method and hepatotoxicity, which is applied in the field of biomedicine and can solve problems such as no clear components
Pending Publication Date: 2021-08-10
GUIZHOU MEDICAL UNIV +1
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However, the material basis of xanthophyll hepatotoxicity is still not clear about the specific components, and the degree of influence of each component on the hepatotoxicity mechanism still needs to be further explored
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[0029] In the following, the technical solution of the present invention will be described in conjunction with examples, but the present invention is not limited to the following examples.
[0030] 1. Extraction of active components of xanthophyll and separation and purification of xanthin B
[0031] Take 9.5 kg of decoction pieces of xanthophyll seeds, add 10 times the volume of distilled water to soak for 1 hour, and then decoct for 1 hour; filter the decoction liquid, add 8 times the volume of distilled water to the filter residue and repeat the decoction and filtration twice, each time for 1 hour. The three extracts were combined, concentrated to 10 L, and 95% ethanol was added to adjust the ratio of ethanol in the extract to 75%, stirred while adding, and then left to stand for 24 hours. After the precipitated solution was suction filtered, the filter cake was washed with 3 times the volume of 75% ethanol, and the filtrates were combined. After recovering the ethanol, pl...
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Abstract
The invention discloses a determination method of hepatotoxic component of airpotato yam. The method comprises the following steps: knocking out xanthotoxin B from all-component extract of airpotato yam, respectively representing the hepatotoxicity of the xanthotoxin B and the hepatotoxicity of the airpotato yam extract from which the xanthotoxin B is knocked out, and determining that the hepatotoxic component of the airpotato yam is the xanthotoxin B and is only the xanthotoxin B. The airpotato yam rhizome extract without the flavanotoxin B has no hepatotoxicity or is relatively weak in hepatotoxicity. The method eliminates the prejudice or misunderstanding of common technicians in the field, including researchers in the field, on the basis of the liver toxic substances of the airpotato yam.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, relates to hepatotoxic components of xanthophyll, in particular to a knock-out control test method for hepatotoxic components of xanthate. Background technique [0002] Huangyaozi is a perennial herb of the Dioscoreaceae Dioscorea genus Huangdu tuber, also known as Huangdu, Huangyaogen, Huangyao, Huangyaozhi, Shanzigu, Jinxiandiaoxia, and iron scales. Mainly produced in Hunan, Hubei, Jiangsu and other places, Shandong, Henan, Anhui, Zhejiang, Fujian and other places are also planted. Huang Yaozi is cool in nature and bitter in taste, and has the effects of cooling blood, reducing fire, clearing gall, and detoxifying. Its pharmacological action and clinical application have been recorded in ancient books, and its medicinal value is high and widely used. In modern clinics, xanthophyll seeds are often used for the treatment of goiter, hematemesis and epistaxis, esophageal cancer, gastric cancer...
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