Ervatamia plant extract as well as extraction and separation method and application thereof

The technology of a plant extract and a separation method is applied in the field of the extract of the genus Bermuda and its extraction and separation, which can solve the problems of single medicinal function and the like, and achieve the effect of inhibiting proliferation

Active Publication Date: 2021-08-13
JINAN UNIVERSITY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] None of the above literatures further enriched and isolated biologi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ervatamia plant extract as well as extraction and separation method and application thereof
  • Ervatamia plant extract as well as extraction and separation method and application thereof
  • Ervatamia plant extract as well as extraction and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the extraction separation method of Bermudagrass extract

[0040] Take 10Kg of dried dog tooth flower (E.divaricata), crush it, extract it by percolation with 10% hydrochloric acid water, combine the extracts, adjust its pH value to 2-3, and then repeatedly extract with chloroform to obtain chloroform layer and acid water respectively. layer. Add ammonia water to the acidic water layer, adjust the pH value to 9-10, and then extract with chloroform to obtain the total alkaloids. Sephadex LH-20 chromatography column for total alkaloids, CH 2 Cl 2 -CH 3 OH (1:1) elution; the first elution fraction was collected, combined for TLC detection, and Bermudagrass extract 1 was obtained.

Embodiment 2

[0041] Embodiment 2: the extraction separation method of Bermudagrass extract

[0042]Take 5 kg of dried leaves of E. officinalis, crush them, extract them by percolation with 95% ethanol, combine the extracts and concentrate under reduced pressure to obtain the total extract. Then add water to suspend, and then add 10% hydrochloric acid to adjust its pH value to 2-3, and repeatedly extract with chloroform to obtain a chloroform layer and an acidic water layer respectively. Add ammonia water to the acidic water layer, adjust the pH value to 9-10, and then extract with chloroform to obtain the total alkaloids. Sephadex LH-20 chromatography column for total alkaloids, CH 2 Cl 2 -CH 3 OH (1:1) elution; the first elution fraction was collected, combined for TLC detection, and Bermudagrass extract 2 was obtained.

Embodiment 3

[0043] Embodiment 3: the extraction separation method of Bermudagrass extract

[0044] Get Hainan dog tooth flower (E.hainanensis) dried whole plant 10Kg, pulverize, use 5% hydrochloric acid water percolation to extract, combine extract, filter, supernatant is loaded on 732 (H + type) cation exchange resin column, followed by water and 0.5mol / L ammonia water for elution, and the ammonia water eluate was collected, CH 2 Cl 2 Extract and evaporate to dryness under reduced pressure to obtain the total alkaloids. Sephadex LH-20 chromatography column for total alkaloids, CH 2 Cl 2 -CH 3 OH (1:1) elution; the first elution fraction was collected, combined for TLC detection, and Bermudagrass extract 3 was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an ervatamia plant extract as well as an extraction and separation method and application thereof. The ervatamia plant extract contains one or more than one bisindole alkaloid selected from bisindole alkaloids E-1 to E-16. The ervatamia plant extract obtained through extraction and separation contains bisindole alkaloid compounds, and can selectively relax pulmonary arteries, inhibit proliferation of pulmonary artery endothelial cells and vascular smooth muscle cells, reduce right ventricular diastolic pressure of pulmonary arterial hypertension mice and inhibit right ventricular hypertrophy. In addition, the series of bisindole alkaloid compounds have chemical structure types different from chemical structure of existing targeted drugs for treating pulmonary arterial hypertension, and the ervatamia plant extract is expected to be developed into novel targeted drugs for treating pulmonary arterial hypertension.

Description

technical field [0001] The invention belongs to the field of plant natural products, and in particular relates to a plant extract of the genus Bermudagrass and its extraction and separation method and application. Background technique [0002] Pulmonary hypertension (Pulmonary hypertension) is a common malignant and progressive pulmonary vascular disease caused by various causes of elevated pulmonary artery pressure, and its pathological features are excessive constriction of pulmonary arterioles and vascular remodeling. Endothelial cell dysfunction is characterized by decreased levels of vasodilation substances such as nitric oxide and prostacyclin, and increased levels of vasoconstrictor substances such as endothelin, resulting in excessive constriction of pulmonary arteries. Vascular remodeling manifests as intimal neogenesis, medial thickening and adventitial fibrosis, etc. The activation and excessive proliferation of endothelial cells and smooth muscle cells are the ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D519/00A61K31/55A61K36/24A61P9/12A61P11/00A61P25/30
CPCC07D519/00A61K36/24A61P9/12A61P11/00A61P25/30C07B2200/07A61K2236/53A61K2236/331A61K2236/333A61K2236/33
Inventor 张晓琦叶文才张冬梅
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap