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Compositions and methods for biodegrading alcohol

A composition and technology of alcohol, applied in the field of compositions and methods for biodegrading alcohol, capable of solving problems such as no provision, accelerated clearance, hypertonic enzyme system, etc.

Pending Publication Date: 2021-08-20
T 巴尔 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the in vivo use of these enzyme systems poses significant problems: the system has a high demand for pyruvate, which is not normally present in large quantities in the gut; a sucrose buffer (50% w / v) is required to stabilize YALDH to Resistance to the action of bile salts; pyruvate and lactate are monovalent ions of salt, yielding two moles of solute per mole of salt; high sucrose concentrations and high salt concentrations lead to hypertonic enzyme systems; and lactic acid produced by circulatory reactions can potentially cause lactic acidosis
Thus, while this approach demonstrates the theoretical feasibility of rapidly oxidatively exsorbing ethanol into the gut using an orally administered enzyme preparation, it does not provide a practical, commercially acceptable solution due to the significant problems enumerated above. means of accelerating the removal of ethanol from the body

Method used

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  • Compositions and methods for biodegrading alcohol
  • Compositions and methods for biodegrading alcohol
  • Compositions and methods for biodegrading alcohol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0186] Example 1: Effect of EtOH dosage (0.05g / ml-0.2g / ml) on open field activities (activity box)

[0187] like figure 1 shown, there is a direct correlation between the amount of alcohol-EtOH administered and the reduced performance of treated mice. 10 min after EtOH IP administration (10 ml / kg solution), 30 min travel distance was measured and compared to individual mouse baseline.

Embodiment 2

[0188] Example 2: Effect of EtOH quenching by alcohol dehydrogenase (KRED) on open field activity (activity box)

[0189] figure 2 The distance traveled in 30 min in the active box test is further illustrated. Specifically, the effect of EtOH (0.5 g / kg) and ADH (10, 100 and 500 mg / kg) (cycles 1 & 2). The distance traveled in the active box test was measured during the 30-minute trial period. ADH used: 500 mg / kg: KRED-P1-A04; 100 / 10 mg / kg: KRED-P1-A12. like figure 2 It is clear that the effect of EtOH on activity is inhibited and / or quenched by KRED.

Embodiment 3

[0190] Example 3: Alcohol dehydrogenase (KRED) administration to EtOH as measured in an open field event (activity box) The quenching effect of and duration of the blocking effect of alcohol dehydrogenase (KRED) on later EtOH administration.

[0191] Table 1, below, summarizes the experimental protocol showing the EtOH effects of KRED inhibition and / or quenching described herein on the CNS and that KRED can prevent the damaging effects of EtOH administration prior to alcohol consumption.

[0192] Table 1

[0193]

[0194]

[0195] image 3 It is further illustrated that the KRED of the present invention not only rescues the destructive CNS effects of EtOH, but also has a preventive-protective effect on the destructive CNS effects of EtOH. Specifically, the ADHs used were KRED-P1-B05 ADH(I) and KRED-P1-B10(ADH II). The active box test shows the total distance traveled (15min). It is important to remind that in image 3 , values ​​are related to wakefulness (less e...

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Abstract

The present invention provides a pharmaceutical composition containing 10 mg to about 100 g KRED and / or a long-acting alcohol dehydrogenase as an active ingredient and a pharmaceutically acceptable carrier. Moreover, provided herein methods for lowering blood alcohol level, methods for preventing a symptom or a risk arising from alcohol consumption and methods for treating a subject afflicted with alcoholism by the administration of the pharmaceutical composition of the invention.

Description

technical field [0001] The present invention relates to; in particular, compositions and methods for removing alcohol in a physiological environment. Background technique [0002] Alcohol dehydrogenases (ADHs) refer to a family of enzymes that catalyze the reversible oxidation of primary or secondary alcohols to aldehydes or ketones. ADH has multiple roles in the body; the main function is to catalyze the oxidation of ethanol (EtOH) to acetaldehyde as the first step in the metabolism of EtOH through the liver, during which it uses NAD+ or NADP+ as electron acceptors. [0003] There are more than 9 different forms of ADH produced by the human body, and each has specific roles in different regions of the digestive tract, although the enzyme has been found to be particularly highly concentrated in the liver and kidneys. The ability to break down alcohol with specific treatments or preparations will be a necessary tool for clinical and pharmacological use. [0004] Alcohol abu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/06C12N9/04A61K38/44C07K19/00
CPCA61K38/00C12N9/0006C12Y101/01C12Y101/01001C07K2319/00C07K2319/31A61P25/32A61K38/443
Inventor T·巴尔T·科瓦里
Owner T 巴尔