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Examples
Experimental program
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Embodiment 1
[0033] Preparation of hsa_circ_0000284 kit for the prognosis of patients with neuroblastoma (20 reactions)
[0046] 13. 10μM hsa_circ_0000284 specific primer 30μl
[0047] Forward primer 5'-TATGTTGGTGGATCCTGTTCGGCA-3', SEQ ID NO:2;
[0048] Reverse primer 5'-TGGTGGGTAGACCAAGACTTGTGA-3', SEQ ID NO:3.
[0049] 14. 10μM GAPDH specific primer 30μl:
[0050] Forward primer 5'-TGCACCACCAACTGCTTAGC-3', SEQ ID NO:4;
[0051] Reverse primer 5'-GGCATGGACTGTGGTCATGAG-3', SEQ ID NO:5.
Embodiment 2
[0053] Detection of hsa_circ_0000284 in tumor tissues and paracancerous tissues of patients with neuroblastoma
[0054] 1. Sample pretreatment: collect the neuroblastoma tissue and paracancerous tissue to be tested respectively, store them in cryopreservation tubes filled with RNA stabilization solution, and put them in a -80°C refrigerator for later use.
[0055]2. Extraction of RNA: Take an appropriate amount of sample, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours to grind the sample, grind it to powder, add 1ml Trizolmortar sample to the mortar, and grind it into a liquid state Afterwards, transfer to the EP tube, add 200 μl / ml Trizol of chloroform to the EP, shake vigorously for 15-30 seconds, let stand on ice for 5 minutes, and centrifuge at 12,000 rpm at 4°C for 15 minutes; carefully take the uppermost aqueous phase and transfer to a new EP tube Add an equal volume of isopropanol, mix it upside down gently, let it stand on ice for 10 minutes; ce...
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Abstract
The invention discloses application of hsa_circ_0000284 in preparation of a neuroblastoma prognostic preparation, and relates to the technical field of molecular biology. The invention further discloses a nucleotide sequence of the hsa_circ_0000284, wherein the nucleotide sequence of the hsa_circ_0000284 is shown as SEQ ID NO:1, and the nucleotide sequences of the specific primers of the hsa_circ_0000284 are shown as SEQ ID NO:2 and SEQ ID NO:3. The survival curve analysis of 38 patients with neuroblastoma shows that the patients with high expression of hsa_circ_0000284 have a relatively poor postoperative survival rate (P is equal to 0.0107). According to the present invention, the expression level of the hsa_circ_0000284 is detected, such that the prognosis judgment is performed on the neuroblastoma patient so as to allow the hsa_circ_0000284 to be used as the important index of the clinical diagnosis evaluation of the neuroblastoma.
Description
technical field [0001] The present invention relates to the technical field of molecular biology, and more specifically relates to the application of hsa_circ_0000284 in preparing neuroblastoma prognostic preparations. Background technique [0002] Neuroblastoma (NB) is the most common extracranial solid tumor in children, originating from the primitive neuroepithelial cells of the embryonic neural crest, and can develop anywhere along the sympathetic nervous system, most commonly in the adrenal medulla. Children with NB account for 8-10% of the number of children with tumors, about 15% of the deaths of children with cancer, and about 50% of them have had tumor metastasis at the time of diagnosis. Early NB can regress spontaneously or simple surgical resection can achieve a good prognosis, but despite intensive treatment measures, the five-year survival rate of children with advanced NB is still less than 40%. To determine the prognosis of patients with neuroblastoma in ord...
Claims
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