Algal extract for use in the treatment or prevention of post-traumatic immunosuppression
An algae extract, immunosuppressive technology, applied in the direction of medical raw materials derived from algae, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc. Problems such as decreased ability to secrete pro-inflammatory cytokines
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Embodiment 1
[0218] Example 1 : Preparation of algal extracts used according to the invention
[0219] Algae extract was prepared as described in Example 1 of International Patent Application WO2015071497.
[0220] One metric ton of fresh, raw Ulva-type green algae was rinsed with fresh water and desanded using an algae cleaner.
[0221] Method steps were performed at ambient temperature unless otherwise indicated.
[0222] The algae (1 metric ton of dehydrated algae with 8% dry matter) were then ground into fine particles by an industrial refiner (brand / model: Inotec "I 175CD-75D"). The term "fine particles" is understood to mean particles with a size in the range of 50 to 1000 nm, there being two populations, the first comprising particle sizes between 50 and 200 nm and the second comprising particle sizes between 600 and 1000 nm.
[0223] The milled material was then pressed using an industrial belt press of make / model Flottweg "B FRU 800HK" at a throughput rate of approximately 1 m...
Embodiment 2
[0230] Example 2 : Studying the Effects of Algae Extracts in a Mouse Model of Traumatic Brain Trauma (Head Trauma) - Materials and Methods
[0231] animal welfare : All experiments were performed in accordance with principles of laboratory animal welfare. All experiments were approved by the Pays de la Loire ethics committee and MESR (Ministère de l’Enseignement supérieur, de la Recherche et de l’Innovation / French Ministry of Higher Education, Research and Innovation) (No. 2016121915529061). Mice (5-week-old Swiss males, strain RjOrl: SWISS and 6-week-old C57BL / 6 males, strain C57BL / 6JRj, weighing 24 to 28 g) were obtained from the Janvier laboratory (Laval). Mice were maintained on a 12-h day / night cycle in the animal facility of the Institut de Recherche en Santé 2 (1RS2) [Institute for Health Research], Nantes, France, with free access to water and food.
[0232] Algae extract including sulfated and non-sulfated polysaccharides : According to the protocol described i...
Embodiment 3
[0250] Example 3 : clinical and bacterial effects
[0251] Mice were divided into 4 groups: sham (S); pneumonia only (PN); traumatic brain trauma+pneumonia (TC+PN); and trauma and infection mice by intraperitoneal injection (IP) every 12 hours 200 μg of compound treatment (TC+PN+TX), starting 2 hours after trauma until euthanasia. Mice in the S and PN groups received a 1 cm incision from the top of the skull without trauma. Pneumonia was induced 24 hours after traumatic brain injury. According to the criteria studied ( figure 1 ), euthanasia occurred 24 to 48 hours after the induction of pneumonia.
[0252] Algal extracts have no effect on weight loss in traumatized and secondary-infected mice
[0253] MSSA pneumonia induced a transient weight loss of approximately 12% of body weight after 24 hours. Cranial trauma increased weight loss at the beginning of infection but did not affect recovery at D+3 compared to non-traumatized mice. Treatment had no effect on weight l...
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Abstract
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