Tissue culture method of paulownia

Abstract

The invention discloses a tissue culture method of paulownia. Raw materials required by the tissue culture method of the paulownia comprise a paulownia plant, a detergent or a saturated clothes washing solution, a 75% alcoholic solution, carbendazim and a culture medium, explants under proper conditions are selected, sterilized and cleaned, then the explants are put into a primary culture medium for induced differentiation, buds generated in the primary culture are put into a proliferation culture medium for proliferation culture, enough proliferation buds are obtained, finally, the obtained proliferation buds are transplanted into a rooting culture medium for rooting, and the rooted plants can be planted in a large scale. According to the tissue culture method of the paulownia, through a plant tissue culture mode, a large number of plants can be cultured within a short time on the basis that few raw materials are used, culture conditions can be artificially and freely created and controlled in the culture process, the culture conditions are not influenced by natural conditions, meanwhile, unified management can be carried out, and automatic control production is facilitated.

Description

technical field [0001] The invention relates to the technical field of woody plant culture, in particular to a tissue culture method of paulownia. Background technique [0002] Paulownia, the genus Paulownia, Scrophulariaceae, aliases: Paulownia white flower, Paulownia large fruit, empty paulownia, etc., the bark is gray, taupe or gray black, smooth when young, longitudinally split when old, false two-branched branches, single leaves, Opposite, large leaves, ovate, entire or lobed, with long stalks, with downy hairs on the stalks, light-loving, shade-tolerant, warm climate-loving, not cold-resistant; Paulownia tree species are all native to China, Young Paulownia grows very fast, it is a fast-growing tree species; Paulownia grows very fast, and the diameter of a ten-year-old Paulownia is twice as large as that of poplar trees of the same age; Paulownia wood has straight texture, uniform structure, no cracking, and is easy to process; air dry Light bulk density, good moistur...