Method for improving purity of japonica rice sterile line
A technology of sterile lines and purity, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problem of seed mixing
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Embodiment 1
[0021] Selected from breeding fields with good natural isolation conditions such as figure 1 For the single plant of Chunjiang 88A shown, plant the seeds of each single plant of Chunjiang 88A in one field, and observe the individual plant morphology of each field, and remove the individual plants with different traits from Chunjiang 88A. It is possible to reduce the degree of influence on the purity of the sterile line and obtain a sterile line with guaranteed purity. The naturally isolated field is separated from other fields by 300 meters to prevent factors such as insects or wind from bringing seeds from other fields into the experimental field and affecting the purity of the seeds. The harvested plants and leaves have no abnormal shape, which is consistent with the traits of Chunjiang 88A. The guaranteed traits are as pure as possible sterile lines, and the seed setting rate is 30%-50%. Too high or too low is not conducive to cultivation, and the sterile line in the non-pa...
Embodiment 2
[0023] The Chunjiang 88A in the above-mentioned Example 1 was replaced with Chunjiang 25A, and the same steps were carried out. By this method, the CMS seeds with a purity higher than 99% could be obtained.
[0024] The traits of Chunjiang 88A and Chunjiang 25A are shown in Table 1 below, and those with different traits from the following traits are out-of-type strains and will be eliminated.
[0025] Table 1
[0026]
[0027] The purity of the sterile line seeds obtained by embodiment 1 and embodiment 2 is all higher than 99% after testing.
[0028] As can be seen from Example 1 and 2 Comparative Example 3, by this method, can obtain the sterile line seed that purity is higher than 99%. The existing CMS breeding methods are generally relatively inefficient, and usually 90% is considered relatively high purity, and the purity effect of 99% cannot be reached. The method is simple and can efficiently obtain high-purity sterile line seeds, and has a promotional effect on var...
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