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Method for improving purity of japonica rice sterile line

A technology of sterile lines and purity, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problem of seed mixing

Pending Publication Date: 2021-09-10
高邮周邶墩农业发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to avoid the mixing of seeds in production. Once other seeds are mixed in, it will snowball. During the continuous multi-generation reproduction process, the proportion of hybrid plants will continue to increase.

Method used

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  • Method for improving purity of japonica rice sterile line
  • Method for improving purity of japonica rice sterile line
  • Method for improving purity of japonica rice sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Selected from breeding fields with good natural isolation conditions such as figure 1 For the single plant of Chunjiang 88A shown, plant the seeds of each single plant of Chunjiang 88A in one field, and observe the individual plant morphology of each field, and remove the individual plants with different traits from Chunjiang 88A. It is possible to reduce the degree of influence on the purity of the sterile line and obtain a sterile line with guaranteed purity. The naturally isolated field is separated from other fields by 300 meters to prevent factors such as insects or wind from bringing seeds from other fields into the experimental field and affecting the purity of the seeds. The harvested plants and leaves have no abnormal shape, which is consistent with the traits of Chunjiang 88A. The guaranteed traits are as pure as possible sterile lines, and the seed setting rate is 30%-50%. Too high or too low is not conducive to cultivation, and the sterile line in the non-pa...

Embodiment 2

[0023] The Chunjiang 88A in the above-mentioned Example 1 was replaced with Chunjiang 25A, and the same steps were carried out. By this method, the CMS seeds with a purity higher than 99% could be obtained.

[0024] The traits of Chunjiang 88A and Chunjiang 25A are shown in Table 1 below, and those with different traits from the following traits are out-of-type strains and will be eliminated.

[0025] Table 1

[0026]

[0027] The purity of the sterile line seeds obtained by embodiment 1 and embodiment 2 is all higher than 99% after testing.

[0028] As can be seen from Example 1 and 2 Comparative Example 3, by this method, can obtain the sterile line seed that purity is higher than 99%. The existing CMS breeding methods are generally relatively inefficient, and usually 90% is considered relatively high purity, and the purity effect of 99% cannot be reached. The method is simple and can efficiently obtain high-purity sterile line seeds, and has a promotional effect on var...

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Abstract

The invention relates to a method for improving the purity of a japonica rice sterile line. The method comprises the following steps: selecting to obtain representative sterile line single plants which have no heterotype in plant leaf morphology, are consistent with a sterile line in a sterile line breeding field, have the maturing rate of 30-50% and are not located at a passage; planting the obtained sterile line single plants and maintainer line rice strains in a naturally isolated field according to a ratio of 6:2-10:2, removing heterotype plants on the plants growing to a vegetative growth period, removing shedding pollen strains and shedding pollen plants in a flowering period, then carrying out artificial pollination, removing the maintainer line after pollination, continuing to culture to a mature period, observing the sterile purity of each panicle, removing strains and heterotype plants of which the plant leaf forms are different from those of sterile line single plants, taking sterile line seeds, carrying out planting, then identifying respective purity, and removing the seeds of which the purity is lower than 99% to obtain the japonica rice with high purity. The method is simple and convenient to operate and high in practicability, and the seed purity of the japonica rice sterile line can be guaranteed.

Description

technical field [0001] The invention relates to a method for improving the purity of japonica rice sterile lines, belonging to the technical field of rice breeding. Background technique [0002] The seed purity of hybrid rice is generally required to be not less than 96%. As the female parent of hybrid rice, the purity of CMS determines the purity of hybrid seeds to a certain extent. In order to ensure the purity of the hybrid, it is necessary to ensure the purity of the sterile line seeds. However, it is difficult to avoid the mixing of seeds in production. Once other seeds are mixed in, it will snowball, and the proportion of hybrid strains will continue to increase in the continuous multi-generation reproduction process. Therefore, in the breeding process of the sterile line, the purity of the provenance must be guaranteed. Also because of this, certain technical measures need to be taken in production to ensure the purity of the sterile line. In recent years, hybrid ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 林建荣仇天松华宇峰石旭东吴明国许会伟施勇烽严昕宋昕蔚孙冰
Owner 高邮周邶墩农业发展有限公司
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