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Application of expression inhibitor of DHX34 gene in preparation of drugs for inhibiting metastasis and invasion of liver cancer cells

A technology of DHX34 and inhibition of liver cancer, applied in the field of biomedicine, can solve the problems of easy metastasis of liver cancer

Active Publication Date: 2021-09-14
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on this, the present invention provides an application of an expression inhibitor of DHX34 gene and / or an inhibitor of DEAH box helicase 34 in the preparation of a drug for inhibiting the metastasis and invasion of liver cancer cells, so as to improve the problem of easy metastasis of liver cancer

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  • Application of expression inhibitor of DHX34 gene in preparation of drugs for inhibiting metastasis and invasion of liver cancer cells
  • Application of expression inhibitor of DHX34 gene in preparation of drugs for inhibiting metastasis and invasion of liver cancer cells
  • Application of expression inhibitor of DHX34 gene in preparation of drugs for inhibiting metastasis and invasion of liver cancer cells

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specific Embodiment

[0062] The following will be described in detail in conjunction with specific embodiments. The following examples do not include other components except unavoidable impurities unless otherwise specified. The reagents and instruments used in the examples are all conventional choices in the art unless otherwise specified. The experimental methods for which specific conditions are not indicated in the examples are implemented according to conventional conditions, such as the conditions described in literature, books or the method recommended by the manufacturer. In the following E7107 is:

[0063]

Embodiment 1

[0065] (1) Using CRISPR / Cas9 technology to knock out the DHX34 gene in human liver cancer cell MHCC97H (from Zhongshan Hospital affiliated to Fudan University) to obtain two cell lines MHCC97H-DHX34 KO1 and MHCC97H-DHX34 KO2, and use Western blot to verify DHX34 Knockout of the gene, the results are as follows figure 1 as shown, figure 1"Parantal" refers to the human liver cancer cell MHCC97H group, and the two bands appearing in the human liver cancer cell MHCC97H group are two subunits of DHX34; "DHX34-KO1" refers to the human liver cancer cell MHCC97H-DHX34 KO1 group; "DHX34 -KO2" refers to the MHCC97H-DHX34KO2 group of human liver cancer cells.

[0066] Depend on figure 1 It can be seen that two stable cell lines MHCC97H-DHX34 KO1 and MHCC97H-DHX34 KO2 were successfully constructed by using CRISPR / Cas9 technology to knock out the DHX34 gene.

[0067] (2) Human liver cancer cells MHCC97H, MHCC97H-DHX34 KO1 and MHCC97H-DHX34 KO2 were cultured in DMEM containing 10% fetal ...

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Abstract

The invention relates to application of an expression inhibitor of a DHX34 gene in preparation of drugs for inhibiting metastasis and invasion of liver cancer cells. Discovered by researches, the DHX34 gene participates in regulating and controlling the metastasis and invasion of the liver cancer cells, and compared with the liver cancer cells without knockout of the DHX34 gene, the metastasis and invasion ability of the liver cancer cells without the DHX34 gene is obviously weakened after the DHX34 gene is knocked out. By applying the expression inhibitor of the DHX34 gene to the preparation of the drugs for inhibiting the metastasis and invasion of the liver cancer cells, the problem that a liver cancer is prone to metastasis can be solved, and the application has an important research significance in further improving the long-term life quality of liver cancer patients.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of a DHX34 gene expression inhibitor in the preparation of drugs for inhibiting the metastasis and invasion of liver cancer cells. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in the world. In the past two decades, the annual mortality rate related to liver cancer has increased significantly, seriously threatening people's health. According to global cancer statistics, primary liver cancer is the sixth most commonly diagnosed cancer, fifth among cancers in men and ninth in women. In addition, primary liver cancer is the fourth leading cause of cancer death worldwide. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma and other rare types, among which, hepatocellular carcinoma is the most common type of liver cancer, accounting for 75% to 85% of all cases, with more than 600,000 ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/496A61K31/365A61K31/35A61K31/352A61P1/16A61P35/00A61P35/04
CPCA61K45/00A61K31/496A61K31/365A61K31/35A61K31/352A61P1/16A61P35/00A61P35/04
Inventor 徐勇周洪钟顾大勇
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN