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Bacillus sonorensis and application thereof

A technology of bacillus and plant fungicide, applied in the field of microorganisms, can solve the problem of single action mechanism of bio-control bacteria

Active Publication Date: 2021-09-14
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, the existing bio-control bacteria have a single mechanism of action. Although as a typical bio-control bacterium, Bacillus has multiple bio-control mechanisms, most of them still focus on competition and the production of antibacterial substances, and few have Reports of Bacillus strains that induce resistance

Method used

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  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof
  • Bacillus sonorensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and identification of Bacillus desertis strain PCYB09

[0023] Pick hickory leaves from Lin’an area, take 3-5 pieces of hickory leaves, wash them under running water, absorb the residual water on the surface with toilet paper, soak them in 70% alcohol solution for 30 seconds, wash them with sterile water for 3 times, and use a homogenizer at low speed Homogenate, take 100μl homogenate and spread on 9cmNA (polypeptone 5g, yeast powder 1g, beef extract 3g, sucrose 15g, NaOH to adjust the pH value to 7.0, add 20g agar powder, sterilize at 121°C for 20 minutes) plate , placed in a 25°C incubator until the colonies grow out, and a single colony is selected and transferred to a new 4cm NA plate. Taking Hickory and Caryaillinoensis as targets, antagonistic strains were screened by confrontation culture method, and the strain with the strongest antagonistic effect was selected and named strain PCYB09.

[0024] Drop sterilized water on the clean cover glass...

Embodiment 2

[0036] Embodiment 2: the fermentation medium, fermentation method and bacterial agent preparation method of Sonora desert bacillus bacterial strain PCYB09

[0037] In order to improve fermentation efficiency while reducing costs, the present invention creates and improves the fermentation medium formula for the strain: the formula of each liter of fermentation medium consists of: 10-20 grams of sucrose, 1-2 grams of yeast powder, beef extract 3-5 grams, 5-10 grams of peptone, 1 gram of potassium phosphate, 0.5 grams of magnesium chloride, 0.1 grams of calcium chloride, 0.01 grams of ferric chloride, 0.5 grams of sodium chloride, and adjust the pH to 5.5-6.5.

[0038]The specific fermentation method is: PCYB09 strain is inoculated with NB liquid medium (5g of polypeptone, 1g of yeast powder, 3g of beef extract, 15g of sucrose) at 37°C for 6-8h, which is the seed liquid, and the seed liquid is prepared according to the ratio of 0.5-1:1000 Ratio, added to the fermentation medium,...

Embodiment 3

[0040] Example 3: Antagonistic ability of Sonora desert bacillus strain PCYB09 to commonly used pathogenic bacteria

[0041] The inhibitory effect of PCYB09 strain on various forest and field diseases harmful to hickory, bamboo, camellia oleifera and rice was tested by confrontation culture method. The specific method is as follows: PCYB09 strain is inoculated with NB medium and shaken at 37°C until it reaches the logarithmic growth phase. Take 3 μL of the bacterial liquid and place it on the PDA plate at a distance of 1.5 cm from the midpoint. Place a 0.5cm-diameter mycelium block at 1.5cm on the other side of the midpoint, press lightly with an inoculation needle so that the mycelium surface is close to the PDA plate, and cultivate in the dark at 28°C until the radius of the colony at the non-confrontation end of the pathogen exceeds 3cm. Measure the growth length of the pathogenic bacteria at the opposite end to the direction of the biocontrol bacteria and the distance betw...

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Abstract

The invention relates to bacillus sonorensis and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides the bacillus sonorensis PCYB09, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 21571; and on the other hand, the invention provides application of the bacillus sonorensis PCYB09. The strain disclosed by the invention shows strong indoor antagonism and field biocontrol effects on various rice and pecan diseases, and also shows strong plant resistance inducing capability and relatively strong ultraviolet resistance, which is innovative academic discovery, and also has higher application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Sonora desert bacillus and an application thereof. Background technique [0002] Bacillus spp. is a class of gram-positive bacteria that can produce spores and is widely distributed in nature. Most bacillus are not pathogenic to animals and plants, and many of them have the activity of inhibiting animal and plant pathogens, and can produce a variety of enzymes, antibiotics and other biologically active products, so they are widely used in industry and agriculture. As early as 1879, Friedrich-Bayer developed a strain of Bacillus (Bacillus subtilis) into a bacterial fertilizer product, which can improve plant nutrition, promote crop health, and increase yield by up to 40%. Since then, new Bacillus strains have been developed and utilized continuously. Since the middle of the last century, the bacillus fungicides developed in the United States have been regist...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00C12R1/07
CPCC12N1/20A01N63/22Y02A40/20
Inventor 张亚波张威滕莹王迪高岩李志红舒金平王浩杰
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY