Rapid typing identification method of lycium barbarum S gene based on targeted sequencing

Abstract

The invention relates to a rapid typing identification method of lycium barbarum S genes based on targeted sequencing. The rapid typing identification method comprises the following steps of designing probes according to S genes of a plurality of reference lycium barbarum varieties, carrying out hybrid capture and sequencing analysis on genes of a lycium barbarum sample to be detected by utilizing a designed probe composition, and comparing a sequencing result with the S genes of the referencelycium barbarum varieties, and judging whether the S gene of the to-be-detected lycium barbarum sample is matched with the S gene of the reference lycium barbarum varieties, and determining the reference lycium barbarum varieties matched with the S gene of the to-be-detected lycium barbarum sample. Specific amplification primers are designed for a plurality of SNP loci to be detected, and the rapid typing identification of the lycium barbarum S-RNase gene is realized by using a genotyping by target sequencing (GBTS) technology.

Description

technical field [0001] The invention belongs to the field of molecular plant breeding, and in particular relates to a method for rapid typing and identification of Lycium barbarum S gene based on targeted sequencing. Background technique [0002] Lycium barbarum (Lycium barbarum L.) belongs to the genus Lycium barbarum in the family Solanaceae. It is a special economic crop with high economic benefits and a wide range of uses. There is a wide range of self-incompatibility in Lycium barbarum, and its compatibility characteristics will have a huge impact on the high and stable yield of Lycium barbarum. The self-incompatibility trait of Lycium barbarum is mainly controlled by the S-RNase (S) gene, most of the clones have two genotypes, and homotypic combination shows self-incompatibility. Accurate understanding of germplasm S genes can help guide breeding practices and pollination tree configuration, and avoid the problem of failure to produce fruit due to self-incompatibility...

AI Technical Summary

Problems solved by technology

The cost of opening a solid-state chip for the first time is more than 500,000 yuan. After a new gene is discovered, it is difficult to add new detection object information, and professional testing equipment is required in the later stage. The cost of a single sample detection is as high as 100 yuan or more.