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Use of bacillus coagulans JA845 in preparing enzyme activity inhibitor with hypoglycemic function

A technology of Bacillus coagulans and enzyme activity inhibitor, which can be used in medical preparations containing active ingredients, pharmaceutical formulas, metabolic diseases, etc., and can solve problems such as no preventive measures.

Pending Publication Date: 2021-09-24
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex pathogenesis and numerous influencing factors of the disease, there is currently no effective control measure

Method used

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  • Use of bacillus coagulans JA845 in preparing enzyme activity inhibitor with hypoglycemic function
  • Use of bacillus coagulans JA845 in preparing enzyme activity inhibitor with hypoglycemic function
  • Use of bacillus coagulans JA845 in preparing enzyme activity inhibitor with hypoglycemic function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Bacillus coagulans JA845 inhibits the activity of α-glucosidase

[0033] 1. Activation of strains and sample preparation

[0034] 1. Activation of strains

[0035] Inoculate the lyophilized powder of Bacillus coagulans JA845 stored at -80°C into LB liquid culture medium, and culture with shaking at 45°C and 180r / min for 24 hours; inoculate the above-prepared fermentation broth into LB liquid culture medium according to the inoculation amount of 3%. Medium, 45 ℃, 180r / min shaking culture for 24h, continuous culture for 3 times, to obtain a highly active fermented seed liquid.

[0036] 2. Sample preparation

[0037] The Bacillus coagulans JA845 fermented seed solution prepared above was inoculated into 100 mL LB liquid medium at an inoculation amount of 3%, and cultured with shaking at 45° C. and 180 r / min for 24 hours to obtain a fermentation liquid.

[0038] (1) Preparation of fermentation supernatant: centrifuge the above-prepared fermentation broth at 4°C...

Embodiment 2

[0055] Example 2 Bacillus coagulans JA845 inhibits the activity of DPP-4

[0056] 1. Activation of strains and sample preparation

[0057] 1. Activation of strains

[0058] Inoculate the lyophilized powder of Bacillus coagulans JA845 stored at -80°C into LB liquid culture medium, and culture with shaking at 45°C and 180r / min for 24 hours; inoculate the above-prepared fermentation broth into LB liquid culture medium according to the inoculation amount of 3%. Medium, 45 ℃, 180r / min shaking culture for 24h, continuous culture for 3 times, to obtain a highly active fermented seed liquid.

[0059] 2. Sample preparation

[0060] The Bacillus coagulans JA845 fermented seed solution prepared above was inoculated into 100 mL LB liquid medium at an inoculation amount of 3%, and cultured with shaking at 45° C. and 180 r / min for 24 hours to obtain a fermentation liquid.

[0061] (1) Preparation of fermentation supernatant: centrifuge the above-prepared fermentation broth at 4°C and 600...

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Abstract

The invention discloses use of bacillus coagulans JA845 in preparing an enzyme activity inhibitor with a hypoglycemic function and relates to the field of hypoglycemic drugs. The bacillus coagulans JA845 is prepared into a fermentation supernatant, a cell-free extract and a bacterial cell metabolite, activity inhibition rates of the bacillus coagulans JA845 to alpha-glucosidase and DPP-4 are measured. Results show that a to-be-detected sample of the bacillus coagulans JA845 has an activity inhibition effect on the alpha-glucosidase and the DPP-4, the cell-free extract of the bacillus coagulans JA845 had the highest inhibition activity, such that the bacillus coagulans JA845 and / or derivatives thereof can be used for preparing the activity inhibitor of the alpha-glucosidase and the DPP-4 and a new thought is provided for preparing hypoglycemic drugs.

Description

technical field [0001] The invention relates to the technical field of hypoglycemic drugs, in particular to the application of bacillus coagulans JA845 in the preparation of an enzyme activity inhibitor with hypoglycemic function. Background technique [0002] Diabetes is a chronic metabolic disease caused by insulin deficiency, insulin resistance, or both. Type 2 diabetes patients are the most at present, accounting for about 85% to 95% of the total number of patients. According to the World Health Organization, the number of adults with diabetes worldwide reached 415 million in 2015 and is expected to reach 642 million by 2040. In addition, diabetic patients are gradually becoming younger. The global incidence of juvenile diabetes in 2017 was 8%, and it is expected to reach 10% by 2040. China has become the country with the largest number of diabetic patients in the world, with more than 100 million diabetic patients. Due to the complex pathogenesis and numerous influen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61P3/10
CPCA61K35/742A61P3/10
Inventor 赵玉娟杨舸李盛钰赵子健王超高磊牛春华
Owner JILIN ACAD OF AGRI SCI
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