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A kind of xanthine oxidase inhibitor and its screening method and application

A technology of xanthine oxidase and menthatriene, which is applied in the field of medicine and can solve problems such as complex chemical components

Active Publication Date: 2022-05-06
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical composition of the sunflower disk extract is complex, and there is no research on the chemical composition of the sunflower disk extract as uric acid-lowering and anti-gout active substances

Method used

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  • A kind of xanthine oxidase inhibitor and its screening method and application
  • A kind of xanthine oxidase inhibitor and its screening method and application
  • A kind of xanthine oxidase inhibitor and its screening method and application

Examples

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preparation example Construction

[0031] In the present invention, the sunflower plate volatile oil is preferably self-made, and the preparation method of the sunflower plate volatile oil preferably includes the following steps: mixing sunflower plate powder, sodium chloride and water, and extracting to obtain the sunflower plate volatile oil.

[0032] In the present invention, the mass ratio of the sunflower disc powder to sodium chloride is preferably 1:0.5-1, more preferably 1:0.75; the mass ratio of the sunflower disc powder to water is preferably 1:10-20, More preferably 1:15. In the present invention, the sunflower disc powder is preferably obtained by crushing sunflower discs and then drying; the present invention has no special limitation on the source of the sunflower discs, and the sunflower discs well-known to those skilled in the art can be used, specifically such as derived from edible sunflower discs At least one of LD5009, edible sunflower SH363 and oil sunflower S606; the present invention has ...

Embodiment 1

[0048] (1) Extraction of volatile oil from sunflower dish

[0049] The sunflower disc of Shikui LD5009 was crushed and then dried to obtain sunflower disc powder. Put 100g of sunflower disc powder into a 2000mL beaker, add 1500mL of water and 75gNaCl, place the beaker in an ultrasonic pot for ultrasonication for 1h, transfer it to a volatile oil extractor, and distill and extract for 8h to obtain the extract; After ether extraction, the gained organic phase was dried with sodium sulfate and then concentrated by a rotary evaporator to remove petroleum ether to obtain the volatile oil of LD5009 sunflower dish (brownish yellow), and the extraction rate of volatile oil of LD5009 sunflower dish was 0.42%. Store in refrigerator at 4°C until use.

[0050] (2) Identification of chemical components of volatile oil from sunflower pan

[0051] The sunflower disc volatile oil was diluted with n-hexane, and then analyzed by GC-MS with Agilent5975 (Agilent, USA). Among them, the conditio...

Embodiment 2

[0053] Prepare sunflower plate volatile oil according to the method of Example 1 and analyze and identify its chemical composition. The difference from Example 1 is that the food sunflower LD5009 is replaced by food sunflower SH363, and the chemical composition analysis results of the food sunflower SH363 sunflower plate volatile oil are as follows figure 2 and shown in Table 1.

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Abstract

The invention provides a xanthine oxidase (XO) inhibitor and an extraction method and application thereof, belonging to the technical field of medicine. The xanthine oxidase inhibitor provided by the present invention includes at least two of zelan chromogen, cumyl alcohol and 1,5,8-p-menthtriene. The XO inhibitor provided by the present invention can form Pi-alkyl and Pi-Pi conjugated double bonds with the active center of XO, and zelan chromogen and cumyl alcohol can form van der Waals force with the active center of XO, and interact with the above-mentioned molecules. In the presence of force, the XO inhibitor is inserted into the hydrophobic gap of XO to occupy its catalytic active center, thereby hindering the entry of the substrate and causing it to lose its original function, which has the effect of inhibiting the production of uric acid. Chromene, cumyl alcohol and 1,5,8-paramenthtriene have the function of inhibiting XO. It provides a new idea for screening XO inhibitors from natural products to prepare drugs for XO-mediated diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a xanthine oxidase inhibitor and its screening method and application. Background technique [0002] Gout is a metabolic disease caused by purine metabolism disorder or excessive production of uric acid in the body and decreased excretion in vitro. Clinical manifestations include hyperuricemia, gouty arthritis, uric acid stones, gout stone formation, etc. Hyperuricemia is a stage in the development of gout; gouty arthritis is a series of inflammatory reactions caused by the deposition of uric acid in joint tissues after the serum uric acid level increases. [0003] The main cause of gout is: when the production of uric acid in the body increases or the excretion decreases, it can lead to an increase in the level of uric acid. When it exceeds its dissolution limit, uric acid will be deposited in the joints and soft tissues, causing an inflammatory response. Uric acid is the fin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/045A61K31/015A61P19/06A61P3/10A61P35/00G01N30/02G01N30/72A61K36/28
CPCA61K31/352A61K31/045A61K31/015A61P19/06A61P3/10A61P35/00G01N30/02G01N30/72A61K36/28
Inventor 韩璐刘新胜韩葳葳董占德高波李婉南
Owner JILIN UNIV
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