Methods, devices and storage media for screening immune repertoires of sequencing biomarkers

Abstract

The present disclosure relates to a method, computing device and storage medium for screening immune repertoire sequencing biomarkers. The method includes: determining the initial absolute abundance of each clone in the sample; calculating the first relative abundance of the cloned genes in the sample based on the initial absolute abundance of the cloned genes in the sample and the sum of the absolute abundances of all clones in the sample In response to determining that the initial absolute abundance of the cloned gene is greater than the predetermined threshold, the first absolute abundance of the cloned gene is determined to be 1; based on the first absolute abundance of the cloned gene in the sample, the first absolute abundance of all clones in the sample A second relative abundance of cloned genes within the sample is calculated; a first test and a second test are performed on candidate cloned genes to determine immune repertoire sequencing biomarkers, and the second test is different from the first test. The present disclosure can significantly improve the reliability of the biomarkers of the screened immune repertoire.

Description

technical field [0001] The present disclosure relates generally to biological information processing, and in particular, to methods, devices, and storage media for screening immune repertoires for sequencing biomarkers. Background technique [0002] In an individual organism, at any given time, the sum of all functionally diverse B cells and T cells in the circulatory system is called the immune repertoire (IR). T cells and B cells, as the main lymphocytes in organisms (such as the human body), are responsible for cellular immunity and humoral immunity, respectively. T cell receptors (TCR) and B cell receptors (BCR) are composed of multiple peptide chains and have antigens. Binding specificity, the amino acid composition and sequence of the complementarity determining region (CDR, also known as hypervariable region) of each peptide chain are highly diverse, constituting a huge library of T cell receptors and B cell receptors, and studies have shown that the subtypes The mor...

AI Technical Summary

Problems solved by technology

[0004] Traditional methods for screening immune repertoire sequencing biomarkers, for example, mainly rely on clonal diversity at the macro level to distinguish disease detection, progression, and prognosis, and are deficient in discovering dominant clones that perform major immune functions In addition, the traditional method for screening immune repertoire sequencing biomarkers is also, for example, using the t test, which does not consider the impact of the type of cloned gene on the significant difference; therefore, the reliability of the screening results is likely to be low

[0005] In summary, the traditional method for screening immune repertoire sequencing biomarkers has the disadvantage of low reliability of the screened immune repertoire biomarkers

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