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Bi-specific extracellular matrix binding peptides and methods of use thereof

An amino acid and sequence technology, applied in the field of cancer treatment and molecular medicine, can solve difficult problems and other problems

Pending Publication Date: 2021-10-08
UNIV OF TARTU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, targeting FN-EDB and TNC-C has proven difficult

Method used

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  • Bi-specific extracellular matrix binding peptides and methods of use thereof
  • Bi-specific extracellular matrix binding peptides and methods of use thereof
  • Bi-specific extracellular matrix binding peptides and methods of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0558] Example 1: Selection and Characterization of PL1 and Related Peptides

[0559] A. Materials and methods

[0560] 1. Materials

[0561] Phosphate-buffered saline (PBS) was purchased from Lonza (Verviers, Belgium), K 3 [Fe(CN) 6 ], HCl, Nuclear Fast Red, Xylene Substitute, Isopropanol, Triton-X, Tween 20, CHCl 3 , MeOH and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Munich, Germany). Cloning, expression, purification of proteins (FN-EDB, TNC-C, NRP1, NCL, and single-chain antibodies FN-EDB-L19 and TNC-C-G11), and generation of polyclonal rabbit antibodies are described below and in Table 1 .

[0562] Table 1: List of antibodies used

[0563]

[0564]

[0565] 2. Cell lines

[0566] U87MG GBM and PC3 prostate cancer cells were obtained from ATCC, and NCH421K cells were obtained from CLS Cell Lines Service GmbH (Eppelheim, Germany). WT-GBM and VEGF-KO GBM cells were a gift from Gabriele Bergers (Leuven, Belgium).

[0567] 3. Clinical samples

...

Embodiment 2

[0659] Example 2: Selection and Characterization of PL2 Peptides and Related Peptides

[0660] Peptides were generally selected and tested for binding to FN-EDB using techniques and protocols as described in Example 1. The data and results are shown in Figures 3 to 5 and 22 in.

[0661] A. Materials and methods

[0662] 1. Materials

[0663] Phosphate buffered saline (PBS) was purchased from Lonza (Verviers, Belgium). K 3 [Fe(CN) 6 ], HCl, Isopropanol, Triton-X, Tween 20, CHCl 3 , MeOH, isopropyl β-D-1-thiogalactoside (IPTG) and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Munich, Germany).

[0664] 2. Peptides and proteins

[0665] Cys-5(6)-carboxyfluorescein (FAM)-PL2 with a 6-aminocaproic acid spacer and Cys-FAM peptide were purchased from TAGCopenhagen (Denmark). Plasmids pASK75-Fn7B8 and pASK75-Fn789 were kindly provided by Dr. Ame Skerra (Schiefner et al., 2012). Amplify the gene fragment of the Fn-EDB domain from a plasmid and clone it into the N-...

Embodiment 3

[0703] Example 3: Selection and Characterization of PL3 Peptides and Related Peptides

[0704] Peptides were generally selected and tested for binding to TNC-C using techniques and protocols as described in Example 1. Some of the data and results are shown in Figures 6 to 8 middle.

[0705] A. Materials and methods

[0706] 1. Materials

[0707] Phosphate buffered saline (PBS) was purchased from Lonza (Verviers, Belgium). K 3 [Fe(CN) 6 ], HCI, Isopropanol, Triton-X, Tween 20, CHCl 3 , MeOH, isopropyl β-D-1-thiogalactoside (IPTG) and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Munich, Germany). Cys-fluorescein (FAM)-PL3 and Cys-FAM peptides with a 6-aminocaproic acid spacer were purchased from TAG Copenhagen (Denmark). Synthesized on a microwave-assisted automated peptide synthesizer (Liberty; CEM Corporation, Matthews, NC, USA) using Fmoc / t-Bu chemistry [ D (KLAKLAK) 2 ]with[ D (KLAKLAK) 2 ] - PL3 peptide (SEQ ID NO: 6). Peptides were purified to 9...

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Abstract

Disclosed are compositions, compounds, and methods relating to peptides that can target and home to cancer, tumors, and extracellular matrix. This is based on the discovery of peptides that can specifically bind to fibronectin extra domain B (FN-EDB), tenascin-C C domain (TNC-C), or both.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of and priority to U.S. Provisional Application No. 62 / 800,879, filed February 4, 2019, which is hereby incorporated by reference in its entirety. [0003] References to Sequence Listings [0004] Pursuant to 37 C.F.R. §1.52(e)(5), hereby incorporated by reference, the Sequence Listing, filed February 4, 2020, is a text file named "TARTU_100_PCT_ST25.txt," created in 2020 January 6th, and is 19,915 bytes in size. technical field [0005] The present invention relates generally to the fields of molecular medicine, cancer therapy, and more specifically, to cell and tissue targeting peptides. Background technique [0006] Targeting anticancer drugs with affinity ligands, such as antibodies and homing peptides, is widely used to strike a balance between increasing drug concentrations at tumor sites and reducing systemic exposure (Kennedy et al., Pharmacol.Ther. (2017), doi: 10.1016 / j....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/00C07K7/08A61K38/04A61K38/16
CPCC07K7/08C07K7/06A61K49/0032A61K49/0093A61K49/0056A61K38/00A61K9/0019A61P35/00C07K14/00
Inventor 坦贝特·蒂萨鲁普拉卡什·林加萨米
Owner UNIV OF TARTU
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