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A kind of anti-inflammatory traditional Chinese medicine composition and application thereof

A kind of composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition

Active Publication Date: 2022-07-05
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rhinitis is a disease with a high incidence rate, but there is no better treatment at present

Method used

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  • A kind of anti-inflammatory traditional Chinese medicine composition and application thereof
  • A kind of anti-inflammatory traditional Chinese medicine composition and application thereof
  • A kind of anti-inflammatory traditional Chinese medicine composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of traditional Chinese medicine composition

[0045] 1. Preparation of No. 1 anti-inflammatory traditional Chinese medicine composition

[0046] Crush hemp seeds, dried ginger, dried tangerine peel, and Daqingye into 80-100 mesh powder, mix 2.5 g of hemp seeds, 2.5 g of dried ginger, 2.5 g of dried dried orange peel, and 2.5 g of Daqingye to obtain a mixture powder, add 150 mL of 90% ethanol aqueous solution (135mL absolute ethanol added 15mL deionized water, shake well), standard Soxhlet extraction for 3h, Soxhlet extraction temperature 78.5 ℃ drying for 20min, collect about 15mL of extract, the extract is concentrated at 45 degrees to volume It is 2-3 mL, transferred to a freeze dryer for 24 hours under reduced pressure and freeze-dried to prepare an extract freeze-dried powder, which is the No. 1 anti-inflammatory traditional Chinese medicine composition.

[0047] 50 mg of the anti-inflammatory traditional Chinese medicine composition was weighed and dis...

Embodiment 3

[0057] Effects of No. 1 anti-inflammatory traditional Chinese medicine composition on the expression of inflammatory cytokines IL-6 and TNF-α

[0058] Using qPCR technology to detect the effect of No. 1 anti-inflammatory traditional Chinese medicine composition in Example 1 on the expression of inflammatory cytokines IL-6 and TNF-α, the specific steps include the following steps:

[0059] 1. THP1 cells (National Experimental Cell Resource Sharing Service Platform) were plated in a 12-well plate at 400,000 / well, and 100ng / mL PMA (Sigma) was added to treat the cells for 24h to induce the cells to become macrophages. The suspended state is transformed into an adherent state.

[0060] 2. The experiment consisted of 3 groups, namely induction + anti-inflammatory group, control group and induction group. Each group was set up with 5 biological repetitions without technical repetitions.

[0061] Induction + anti-inflammatory group (Formula): to part of the cell suspension in step 1...

Embodiment 4

[0073] Effects of No. 1 anti-inflammatory traditional Chinese medicine composition on the expression of inflammatory cytokines IL-6 and CCL2

[0074] Using qPCR technology, the effect of the anti-inflammatory traditional Chinese medicine composition in Example 1 on the expression of inflammatory cytokines IL-6 and CCL2 was detected, which specifically included the following steps:

[0075] 1. HMC-1 cells were plated at 300,000 / well in a 12-well plate to obtain a cell suspension;

[0076] 2. The experiment consisted of 3 groups, namely induction + anti-inflammatory group, control group and induction group. There were 5 biological replicates in each group.

[0077] Induction + anti-inflammatory group (Formula): add the anti-inflammatory traditional Chinese medicine composition mother solution of Example 1, PMA and A23187 (Abcam, used to stimulate inflammation) to part of the cell suspension in step 1 to make the anti-inflammatory traditional Chinese medicine composition. The c...

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Abstract

The invention discloses an anti-inflammatory traditional Chinese medicine composition and an application thereof. The active components of the anti-inflammatory traditional Chinese medicinal composition are prepared from traditional Chinese medicine raw materials, and the traditional Chinese medicinal raw materials are composed of hemp seed, dried ginger, dried tangerine peel and Daqingye. The extract prepared according to a certain proportion can significantly inhibit the expression of inflammatory factors such as IL-6, TNF-а and CCL2, and inhibit the production of vascular endothelial relaxation factor NO. It can inhibit the expression of inflammatory factors in multiple inflammatory cells; it can also inhibit the release of inflammatory mediators.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine compositions, in particular, to an anti-inflammatory traditional Chinese medicine composition and applications thereof. Background technique [0002] Rhinitis is an irritant, or inflammatory, reaction that occurs in the mucous membrane of the nose. Its common symptoms are nasal congestion, runny nose, sneezing and itching. Inflammation is caused by viruses, bacteria, irritants or allergens. The most common form of rhinitis is allergic rhinitis, usually caused by airborne allergens such as pollen and dander. Rhinitis is a high-morbidity disease, but there is currently no better treatment. [0003] During the occurrence of rhinitis, macrophages act as antigen-presenting cells to present allergens, and at the same time, they also secrete a large number of cytokines and chemokines after activation to recruit more inflammatory cells to participate in the pathological process. Cytokines a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9068A61P11/02A61P29/00
CPCA61K36/60A61K36/9068A61K36/752A61K36/855A61K36/704A61K36/315A61K36/195A61P11/02A61P29/00A61K2236/333A61K2236/51A61K2300/00
Inventor 黄文婷谢兰党红蕾郭弘妍邢婉丽程京
Owner CAPITALBIO CORP