Covalent organic framework material, preparation method and application thereof, aptamer sensor and preparation method thereof

A covalent organic framework and aptamer sensor technology, applied in the field of electrochemical sensing, can solve the problems of unsatisfactory sensitivity and cumbersome manufacturing process of electrochemical biosensors

Pending Publication Date: 2021-10-15
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, based on Au nanoparticles, TiO 2 , graphene oxide (GO), and electrochemical G-quadruplex DNA biosensors with materials such as magnetic beads combined with carbon black have been used to detect infected COVID-19 patients, however, the fabrication process of these electrochemical biosensors Cumbersome and unsatisfactory sensitivity

Method used

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  • Covalent organic framework material, preparation method and application thereof, aptamer sensor and preparation method thereof
  • Covalent organic framework material, preparation method and application thereof, aptamer sensor and preparation method thereof
  • Covalent organic framework material, preparation method and application thereof, aptamer sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0070] Preparation of phosphate buffer solution: 8.00g NaCl, 0.20g KCl, 1.44g Na 2 HPO 4 , 1.8g K 2 HPO 4 Dissolve in 800mL ultrapure water (≥18.2MΩ·cm), adjust the pH value of the solution to 7.4 with hydrochloric acid, and finally dilute to 1L with ultrapure water (≥18.2MΩ·cm) to obtain a phosphate buffered saline solution (PBS, 0.01mol / L, pH=7.4).

[0071] Preparation of electrolyte solution: 1.6g K 3 Fe(CN) 6 , 2.1 g K 4 Fe(CN) 6 and 7.5g KCl were added to the above-prepared phosphate buffer solution (PBS, 0.01mol / L, pH=7.4) to obtain an electrolyte solution, which was used for the electrochemical detection of the aptasensor.

[0072] Preparation of N58 aptamer solutions with different concentrations: Add phosphate buffer solution (PBS, 0.01mol / L, pH=7.4) to the stock solution of N58 aptamer, and prepare the concentrations of 1, 10, 50, 100 and 200nmol / L, respectively. L of N58 aptamer solution.

[0073] Preparation of N-gene solutions with different concentration...

Embodiment 1

[0077] The covalent organic framework material of this example consists of 5,10,15,20-tetraamine (4-aminophenyl) porphyrin (TAPP) and 2,2'-bipyridine-5,5'-dicarbaldehyde (BPDA ) is prepared by a Schiff base reaction under the action of a catalyst; wherein, the temperature of the Schiff base reaction is 120°C, the reaction time is 72h, the molar ratio of TAPP and BPDA is 23:41, and the Schiff base reaction The solvent of the alkali reaction is composed of o-dichlorobenzene and n-butanol, the volume ratio of the o-dichlorobenzene and n-butanol is 1:1, and the catalyst is acetic acid.

[0078] Two, the specific embodiment of the preparation method of the covalent organic framework material of the present invention is as follows:

Embodiment 2

[0080] The covalent organic framework material prepared by the method for preparing the covalent organic framework material in this example is the covalent organic framework material in Example 1, and the specific steps are as follows:

[0081]5,10,15,20-tetraamine(4-aminophenyl)porphyrin (TAPP) (15.6 mg, 0.023 mmol) and 2,2'-bipyridine-5,5'-dimethylaldehyde (BPDA) ( 8.7 mg, 0.041 mmol) was added into a Schlenk bottle (15 mL in volume) containing 2.5 ml o-dichlorobenzene and 2.5 ml n-butanol, the solution was dark green at this time, then the Schlenk bottle was placed in an ultrasonic cleaner, and passed Shake ultrasonically for 30 minutes to form a homogeneous solution, and add 0.2 mL of acetic acid solution with a concentration of 6 mol / L as a catalyst. Under the protection of nitrogen, the air in the reaction bottle was removed through three cycles of freezing-pumping and thawing, and the reaction was carried out at a temperature of 120° C. for 72 hours. After the reaction...

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Abstract

The invention relates to a covalent organic framework material, a preparation method and application thereof, an aptamer sensor and a preparation method thereof, and belongs to the technical field of electrochemical sensing. The covalent organic framework material is prepared from tetraaminophenyl porphyrin and dipyridyl dicarboxaldehyde through a Schiff base reaction under the action of a catalyst, and the covalent organic framework material (p-COF) is two-dimensional layered piled nanospheres and has the advantages of large specific surface area, large pore diameter, rough surface, rich functional groups, a highly conjugated network structure and good electrochemical activity. Therefore, a large number of aptamer chains not only can be tightly anchored on the surface of p-COF, but also can permeate into p-COF pores through hydrogen bonds, pi-pi stacking interaction or electrostatic force between positive and negative ions. The covalent organic framework material (p-COF) has strong binding effect with the SARS-CoV-2 antibody and the N-gene targeting aptamer chain, and can be used for rapid and accurate detection of the severe acute respiratory syndrome coronavirus-SARS-CoV-2.

Description

technical field [0001] The invention relates to a covalent organic framework material, a preparation method and application thereof, an aptamer sensor and a preparation method thereof, and belongs to the technical field of electrochemical sensing. Background technique [0002] The rapid global evolution of the novel coronavirus, known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is causing unprecedented impacts on national health systems, science and society. COVID-19 is considered to be the third most pathogenic human coronavirus to emerge in the past 20 years. Currently, the main detection methods for SARS-CoV-2 include polymerase chain reaction (PCR), nucleic acid hybridization technology or immunoassay methods. A variety of immunoassays have been developed for the detection of the COVID-19 virus, such as peptidyl luminescent immunoassays, enzyme-linked immunosorbent assays (ELISA), immunochromatographic assays, and lateral flow immunoassays. Althoug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 崔静何领好薛雨林阚伦张子轩张治红方少明
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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