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Spectral unmixing

A light irradiation and fluorophore technology, applied in the field of spectral decomposition, can solve problems such as interfering samples and sample destruction

Pending Publication Date: 2021-10-15
SARTORIUS BIOANALYTICAL INSTR INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This imaging can include the addition of contrast agents or chemical reagents, which can lead to destruction of the sample
However, this method interferes with the sample being imaged, requiring incubation of multiple sample pools for correspondingly different durations to assess the response of the sample population over time

Method used

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Examples

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Embodiment Construction

[0021] Examples of methods and systems are described herein. It should be understood that the words "exemplary," "example," and "illustrative" are used herein to mean "serving as an example, instance, or illustration." Any embodiment or feature described herein as "exemplary," "example" or "illustrative" is not necessarily to be construed as preferred or advantageous over other embodiments or features. Furthermore, the exemplary embodiments described herein are not meant to be limiting. It should be readily appreciated that some aspects of the disclosed systems and methods can be arranged and combined in many different configurations.

[0022] I. overview

[0023] A contrast agent can be added to the sample to facilitate imaging of the sample. This sample can include a biological sample containing cells of interest. Contrast agents may include fluorescent dyes, non-fluorescent dyes or pigments, nanorods or other conductive elements exhibiting surface plasmon resonance at...

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Abstract

Systems and methods are provided for microscopically and fluorescently imaging cell-hearing biological samples or other samples of interest. A microscope objective or other optical elements that exhibits chromatic aberration can be used to obtain images of fluorophores or other contrast agents at different wavelengths. The obtained images are then used to correct each other, e.g., to remove artifacts in an image of a shorter-wavelength fluorophore that are caused by cross-talk from a longer-wavelength fluorophore. A longer-wavelength image, taken at a focal distance corresponding to the shorter-wavelength fluorophore, is taken and used to subtract the activity of the longer-wavelength fluorophore in the shorter-wavelength image. The longer-wavelength image may be taken using a microscope set to the shorter-wavelength focal distance. Alternatively, the longer-wavelength image may be simulated by applying a blurring filter or other methods to a longer-wavelength image taken when the microscope is set to the longer-wavelength focal distance.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Patent Application Serial No. 16 / 264819, filed February 1, 2019, the entire contents of which are incorporated herein by reference. Background technique [0003] Cells (e.g., cultured cells, transplanted tissue samples) can be incubated in a variety of media and exposed to a variety of conditions (e.g., temperature, dissolved gas levels, radiation, humidity, added substances, electric or magnetic fields, viruses, microbial ) to assess the cellular response to the applied conditions. Can measure the response of cells to applied conditions to assess the efficacy of drugs or treatments, to assess the toxicity of substances (for example, experimental drugs or treatments), to study the effects of genetic modifications of cells, to study the metabolism of cells and / or tissues formed from them omics, structural or other properties, or to determine other information. This evaluation can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6458G01N2021/6419G01N2021/6421G01N21/6428G01N21/658G06T5/50G01N2021/6439G06T2207/10056G06T2207/10064G06T2207/10148G06T2207/10152G06T2207/30024G06T5/90G06T5/70
Inventor P·J·比尔茨N·霍尔茨E·W·恩兹利
Owner SARTORIUS BIOANALYTICAL INSTR INC
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