Unlock instant, AI-driven research and patent intelligence for your innovation.

Methylation of imprinting gene as marker for early diagnosis of cardiovascular and cerebrovascular diseases

A cardiovascular and cerebrovascular disease and methylation technology, applied in the medical field to improve the effect of early diagnosis and treatment

Pending Publication Date: 2021-10-22
南京腾辰生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, abnormal DNA methylation signals in blood may bring breakthroughs in the early diagnosis of cardiovascular and cerebrovascular diseases in vitro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methylation of imprinting gene as marker for early diagnosis of cardiovascular and cerebrovascular diseases
  • Methylation of imprinting gene as marker for early diagnosis of cardiovascular and cerebrovascular diseases
  • Methylation of imprinting gene as marker for early diagnosis of cardiovascular and cerebrovascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] Example 1. Primer Design for Detection of SLC22A18 Gene Methylation Sites

[0136] After extensive sequence and functional analysis, four fragments (SLC22A18_A fragment, SLC22A18_B fragment, SLC22A18_C fragment and SLC22A18_D fragment) in the imprinted gene SLC22A18 (Solute CarrierFamily22, member18, SLC22A18) were selected for correlation between methylation level and cardiovascular and cerebrovascular diseases analyze.

[0137] The SLC22A18_A fragment (SEQ ID No.1) is located in hg19 reference genome chr11:2920020-2920844, positive sense strand.

[0138] The SLC22A18_B fragment (SEQ ID No.2) is located at hg19 reference genome chr11:2921564-2922490, antisense strand.

[0139] The SLC22A18_C fragment (SEQ ID No.3) is located at hg19 reference genome chr11:2924390-2925287, positive sense strand.

[0140] The SLC22A18_D fragment (SEQ ID No.4) is located in hg19 reference genome chr11:2925321-2926242, positive sense strand.

[0141] The CpG site information in the SLC2...

Embodiment 2

[0159] Example 2, SLC22A18 gene methylation detection and result analysis

[0160] 1. Research samples

[0161] The research sample adopts the epidemiological cluster sampling method, and conducts a follow-up survey on the community population over 18 years old in a certain city for more than 2 years. This study was reviewed by the Ethics Committee, and all subjects signed the informed consent. Cardiovascular and cerebrovascular diseases and cancer incidence information are recorded every year through local hospitals, CDC chronic disease management system, community health service centers and workstation chronic disease routine registration items, and social security center reimbursement data. The start time of the cohort is the baseline survey date, and the outcome variable is cardiovascular and cerebrovascular diseases. For the lost follow-up subjects, the follow-up time is uniformly calculated as half of the end time of the follow-up. As of the follow-up date in July 2018...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses methylation of an imprinting gene as a marker for early diagnosis of cardiovascular and cerebrovascular diseases. The invention provides an application of a methylated SLC22A18 gene as a marker in preparation of a product. The application of the product is at least one of the following applications: auxiliary diagnosis of cardiovascular and cerebrovascular diseases or early warning of the cardiovascular and cerebrovascular diseases before clinical symptoms; auxiliary diagnosis of coronary heart disease or early warning of coronary heart disease before clinical symptoms; auxiliary diagnosis of cerebral apoplexy or early warning of cerebral apoplexy before clinical symptoms; auxiliary distinguishing of the coronary heart disease and the cerebral apoplexy; auxiliary diagnosis of coronary heart diseases with different clinical characteristics or early warning of coronary heart diseases with different clinical characteristics before clinical symptoms; auxiliary diagnosis of the cerebral apoplexy with different clinical characteristics or early warning on the cerebral apoplexy with different clinical characteristics before clinical symptoms. The invention has important scientific significance and clinical application value for improving the early diagnosis and treatment effect of cardiovascular and cerebrovascular diseases and reducing the death rate.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the methylation of an imprinted gene as a marker for early diagnosis of cardiovascular and cerebrovascular diseases. Background technique [0002] Cardiovascular and cerebrovascular diseases are collectively referred to as cardiovascular and cerebrovascular diseases. disease. Cardiovascular and cerebrovascular disease is a common disease that seriously threatens the health of human beings, especially middle-aged and elderly people over 50 years old. It has the characteristics of high prevalence, high disability rate and high mortality rate. At present, the number of people dying of cardiovascular and cerebrovascular diseases in the world is as high as 15 million people every year. The morbidity and mortality of cardiovascular and cerebrovascular diseases in my country are generally on the rise. The number of people who die from cardiovascular and cerebrovascular diseases is 3.5 million ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883G16B40/00
CPCC12Q1/6883G16B40/00C12Q2600/154
Inventor 狄飞飞韦玉杰王俊
Owner 南京腾辰生物科技有限公司