Kit and system for evaluating prognosis of glioma and/or gastric adenocarcinoma
A kit and technology for gastric adenocarcinoma, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problem of inaccurate prognostic evaluation and achieve the effect of improving accuracy
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Embodiment 1
[0086] In this example, the cDNA of the glioma sample synthesized in the preparation example was verified by PCR.
[0087] The primers used for PCR verification are the first primer shown in SEQ ID NO:4 and the second primer shown in SEQ ID NO:5. The operation of PCR was carried out according to the instructions of synthetic primers and PCR kit. PCR products are subjected to agarose gel electrophoresis to display the presence or absence of amplified bands, such as figure 1 As shown, the amplified bands that appear are recovered using a DNA gel recovery kit (QIAquick PCR purification kit, purchased from Qiagen), and then subjected to Sanger sequencing. The sequencing results are shown in figure 2 .
[0088] Depend on figure 1 and figure 2 It can be seen that the MET gene with exon 10 deletion exists in the glioma samples.
Embodiment 2
[0090] In this example, the RNA of the glioma sample collected in the preparation example is sequenced.
[0091] The RNA of each sample is constructed RNA library using RNA library construction kit (purchased from Illumina), then use sequencing platform (IlluminaHiSeq 2000) to carry out RNA sequencing to RNA library, and screen out RNA sequencing results containing such as SEQ ID NO:2 The glioma samples of the mRNAs shown are shown in Table 2 for the results.
[0092] Table 2
[0093]
[0094] As can be seen from Table 2, there were 14 cases of glioma samples containing the mRNA shown in SEQ ID NO:2, and the median survival period was 200 days, and the mRNA shown in SEQ ID NO:2 was not detected There are 1197 cases of glioma samples, and the median survival time is 872 days, indicating that the prognosis of glioma containing the mRNA shown in SEQ ID NO: 2 is poor, so it can be used to evaluate the prognosis of glioma.
[0095]In addition, in all-grade glioma, IDH-mutated ...
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