Primer group, probe, kit and detection method for rapidly identifying novel coronavirus

A coronavirus and detection method technology, applied in the field of virus strain identification, can solve the problem of not mentioning the novel coronavirus SARS-CoV-2 detection primer probe set and kit, etc., and achieves convenient operation and strong practicability for clinical application. , a strong specific effect

Pending Publication Date: 2021-10-22
SOUTH CHINA AGRI UNIV
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  • Abstract
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Problems solved by technology

[0008] The above two invention patent applications respectively disclosed the primer probe sets and kits for the detection of coronavirus NL63 by RPA technology and the detection of porcine delta coronavirus by LFD-RPA technology, but neither mentioned the new coronavirus SARS- CoV-2 Detection Primer Probe Sets and Kits

Method used

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  • Primer group, probe, kit and detection method for rapidly identifying novel coronavirus
  • Primer group, probe, kit and detection method for rapidly identifying novel coronavirus
  • Primer group, probe, kit and detection method for rapidly identifying novel coronavirus

Examples

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Embodiment 1

[0049] In the following examples: the RNA extraction kit is the viral nucleic acid RNA extraction kit (article number: 52904) of Qiagen; , its 20μL reaction system: 5×PrimeScript IV cDNA Synthesis Mix 4μL, Random 6mers (50μM) 2μL, template RNA 5μL, RNase Free H 2 O 9 μL, after mixing, react according to the following conditions: 30°C for 10 minutes, 42°C for 20 minutes, and finally 70°C for 15 minutes to inactivate; purchased from TwistDx, UK nfo kit (catalog number: TANFO02KIT); recombinase uvs X, single-strand binding protein and DNA polymerase have been added to the RPA enzyme tube when purchased.

[0050] A kind of primer group and probe that are used to quickly identify novel coronavirus, described primer group and probe are compared the novel coronavirus (SARS-CoV that has been announced in the GenBanK database of NCBI (National Center for Bioinformatics of the United States) in a large number by the inventor -2, the S gene of MN908947.3) and its sequence before and a...

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Abstract

The invention belongs to the technical field of virus strain identification methods, and particularly relates to a primer group, a probe, a kit and a detection method for rapidly identifying novel coronavirus, the nucleotide sequences of the primer group and the probe are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the 5'end of the nucleotide sequence of the downstream primer is provided with biotin, the 5 'end of the nucleotide sequence of the probe is provided with carboxyl fluorescein, the 3' end of the nucleotide sequence of the probe is provided with a C3-spacer group, and the primer group and the probe are high in specificity and sensitivity. The detection method has the advantages of strong specificity, simple operation process, no initial heating step and high sensitivity, and can accurately detect the SARS-CoV-2 in a shorter time.

Description

technical field [0001] The invention belongs to the technical field of virus strain identification methods, and in particular relates to a primer set, a probe, a kit and a detection method for rapidly identifying novel coronaviruses. Background technique [0002] Novel coronavirus pneumonia (COVID-19, NCP) is pneumonia caused by novel coronavirus (Severe Acute Respiratory Syndrome Coronavirus, SARS-CoV-2), clinical manifestations are mainly characterized by fever, fatigue, dry cough, nasal congestion, runny nose, etc. Upper respiratory symptoms are relatively rare. Respiratory droplets and close contact transmission are the main routes of transmission of the disease. There is a possibility of aerosol transmission in a relatively closed environment when exposed to high concentrations of aerosols for a long time. Based on the current epidemiological investigation, the incubation period of the disease is 1 to 14 days, mostly 3 to 7 days. The main manifestations are fever, fatig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/701C12Q1/6844C12Q2531/119C12Q2522/101C12Q2521/507C12Q2565/625C12Q2563/107C12Q2521/107Y02A50/30
Inventor 沈永义陈晓媛
Owner SOUTH CHINA AGRI UNIV
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